H00009241-M04

antibody from Abnova Corporation

Targeting: NOG SYM1, SYNS1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009241-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009241-M04, RRID:AB_581872

Product name

NOG monoclonal antibody (M04), clone 4A1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant NOG.

Antigen sequence

QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLN
ETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGG
AEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQG
KKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSR
FWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTV
LRWRCQRRGGQRCGWIPIQYPIISECKCSC


Isotype

IgG

Antibody clone number

4A1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NOG expression in transfected 293T cell line by NOG monoclonal antibody (M04), clone 4A1.Lane 1: NOG transfected lysate(25.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NOG is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol