TA500028

antibody from OriGene

Targeting: NOG SYM1, SYNS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500028

Provider

OriGene

Proper citation

OriGene Cat#TA500028, RRID:AB_2151687

Product name

Noggin (Noggin) mouse monoclonal antibody, clone OTI4C12 (formerly 4C12)

Antibody type

Monoclonal

Description

Noggin (Noggin) mouse monoclonal antibody, clone OTI4C12 (formerly 4C12)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

NOG

Isotype

IgG

Antibody clone number

OTI4C12

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Noggin antibody (4C12) at 1:2000 + Recombinant human Noggin (Cat# RC205020)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody (TA500028).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-Nog mouse monoclonal antibody (TA500028) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog(RC205020).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500028)

Validation comment

IHC