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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-109231 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Noggin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Noggin Recombinant protein, PC-3M, rat liver tissue, human liver tissue, human placenta tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Noggin in Recombinant protein. Samples were incubated with Noggin polyclonal antibody (Product # PA5-109231), at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Noggin in Recombinant protein. Samples were incubated with Noggin polyclonal antibody (Product # PA5-109231), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Noggin in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Noggin polyclonal antibody (Product # PA5-109231), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Noggin in paraffin-embedded rat liver tissue. Samples were incubated with Noggin polyclonal antibody (Product # PA5-109231), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Noggin in paraffin-embedded human placenta tissue. Samples were incubated with Noggin polyclonal antibody (Product # PA5-109231), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Noggin in paraffin-embedded human liver tissue. Samples were incubated with Noggin polyclonal antibody (Product # PA5-109231), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Noggin in PC-3M cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Noggin polyclonal antibody (Product # PA5-109231) at a dilution of 1:100.
