MA5-56496

antibody from Invitrogen Antibodies

Targeting: KDM2B CXXC2, Fbl10, FBXL10, JHDM1B, PCCX2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-56496

Provider

Invitrogen Antibodies

Product name

FBXL10 Recombinant Rabbit Monoclonal Antibody (PSH05-38)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

PSH05-38

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of FBXL10 with HeLa cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496)at a dilution of 1:2,000 in 1% BSA in PBST overnight at 4 ℃. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of FBXL10 with NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp). Next they were incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496)at a dilution of 1:2,000 in 1% BSA in PBST overnight at 4 ℃. Followed by Goat Anti-Rabbit IgG H&L at a dilution of 1:1,000. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FBXL10 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at 1:1,000 dilution. Followed by Anti-Rabbit IgG for IP Nano-secondary antibody at 1:5,000 dilution (1 hr, room temperature). Lane 1: MDA-MB-231 cell lysate; Lane 2: (MA5-56496) IP in MDA-MB-231 cell lysate; Lane 3: Rabbit IgG in MDA-MB-231 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FBXL10 with paraffin-embedded human colon tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at a dilution of 1:200 (1 hr, room temp).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FBXL10 with paraffin-embedded rat brain tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at a dilution of 1:1,000 (1 hr, room temp).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FBXL10 with paraffin-embedded human brain tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at a dilution of 1:200 (1 hr, room temp).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FBXL10 with paraffin-embedded mouse brain tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at a dilution of 1:1,000 (1 hr, room temp).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FBXL10 with paraffin-embedded mouse skin tissue. The tissues were blocked in 1% BSA (20 min, room temp), washed with ddH2O and PBS, incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at a dilution of 1:200 (1 hr, room temp).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of FBXL10 with NIH/3T3 cells. The cells were fixed, permeabilized and incubated with FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496) at a dilution of 1:1,000 (red) and compared with Rabbit IgG Isotype Control (green). Followed by iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution (30 min, 4℃).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) assay of FBXL10 was performed using FBXL10 Recombinant Monoclonal Antibody (Product # MA5-56496 1 µg) (green) with cross-linked chromatin from MDA-MB-231 cells. Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by real-time PCR using primers. DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin.