65695ACTMOTIF

antibody from Invitrogen Antibodies

Targeting: BCL7A BCL7

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

65695ACTMOTIF

Provider

Invitrogen Antibodies

Product name

Bcl7A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl7 antibody (pAb) tested by Western blot 20 µg of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at a dilution of 1:500. The calcµLated M.W. of Bcl7A is 23 kDa, whereas its observed M.W. on SDS-PAGE is 45 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bcl7 antibody (pAb) tested by Immunoprecipitation. 5 µL of Bcl7A antibody (Lane 1) or an unrelated IgG (Lane 2) was used to immunoprecipitate Bcl7A from 500 µg of HEK293 whole cell extract. The immunoprecipitated protein was detected by Western blotting using a the same Bcl7A antibody at a dilution of 1:1,000 (crossreaction between heavy and light chains of the antibody is observed and is common).