Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-20131 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody recognizes both isoforms of TSC1. A suggested positive control is EL4 cell lysate. PA5-20131 can be used with blocking peptide PEP-0249.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex.
Poulopoulos A, Murphy AJ, Ozkan A, Davis P, Hatch J, Kirchner R, Macklis JD
Nature 2019 Jan;565(7739):356-360
Nature 2019 Jan;565(7739):356-360
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EL4 cell lysate using a TSC1 polyclonal antibody (Product # PA5-20131) in the (A) absence or (B) presence of blocking peptide at a 1 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TSC1 in EL4 cell lysate with TSC1 Polyclonal Antibody (Product # PA5-20131) at 1 µg/mL in the (A) absence and (B) presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of TSC1 in EL4 cells with TSC1 Polyclonal Antibody (Product # PA5-20131) at 2 µg/mL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of TSC1 in L1210 cells with TSC1 Polyclonal Antibody (Product # PA5-20131) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL