24830002

antibody from Novus Biologicals

Targeting: AXIN2 DKFZp781B0869, MGC126582

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

24830002

Provider

Novus Biologicals

Proper citation

Novus Cat#24830002, RRID:AB_2274551

Product name

Rabbit Polyclonal Axin-2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This product is specific for Human AXIN2

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.05 mg

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections with Obesity.

Garrison CB, Lastwika KJ, Zhang Y, Li CI, Lampe PD
Journal of proteome research 2017 Jan 6;16(1):274-287

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High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays.

Rho JH, Lampe PD
Journal of proteome research 2013 May 3;12(5):2311-20

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Axin-2 Antibody [24830002] - Western Blot was performed using affinity purified SEQer Axin-2 Antibody, aa(577-676) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer Axin-2 Antibody, aa(577-676) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Axin-2 Antibody [24830002]