Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [9]
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Validation data
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- Product number
- NBP1-87421 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87421, RRID:AB_11005736
- Product name
- Rabbit Polyclonal Pyruvate Dehydrogenase E1 beta subunit Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Pyruvate Dehydrogenase E1 beta subunit antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Analysis using Anti-PDHB antibody NBP1-87421 (A) shows similar pattern to independent antibody NBP2-38327 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human heart muscle shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining in human heart muscle and pancreas tissues using anti-PDHB antibody. Corresponding PDHB RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human kidney.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human cerebral cortex shows moderate positivity in neuropil.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human cerebral cortex, heart muscle, kidney and pancreas using Anti-Pyruvate Dehydrogenase E1 beta subunit antibody NBP1-87421 (A) shows similar protein distribution across tissues to independent antibody NBP2-38327 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody [NBP1-87421] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in distal tubules.