PA5-27989

antibody from Invitrogen Antibodies

Targeting: PDHB PDHE1B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27989

Provider

Invitrogen Antibodies

Product name

PDHB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, H1299, HCT116, mouse heart, rat heart.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PDHB Polyclonal Antibody (Product # PA5-27989). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. 10% SDS PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PDHB Polyclonal Antibody (Product # PA5-27989). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. 10% SDS PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PDHB Polyclonal Antibody detects PDHB protein by Western blot analysis. A. 50 µg rat heart lysate/extract.10 % SDS-PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) dilution: 1:5,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PDHB Polyclonal Antibody (Product # PA5-27989). Sample (50 µg of whole cell lysate). Lane A: mouse heart. 10% SDS PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PDHB was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PDHB Polyclonal Antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PDHB Polyclonal Antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.