Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27989 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDHB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HCT116, mouse heart, rat heart. Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (85%), Xenopus laevis (84%), Pig (94%), Chicken (85%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PDHB Polyclonal Antibody (Product # PA5-27989). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. 10% SDS PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PDHB Polyclonal Antibody (Product # PA5-27989). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. Lane C: HCT116. 10% SDS PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PDHB Polyclonal Antibody detects PDHB protein by Western blot analysis. A. 50 µg rat heart lysate/extract.10 % SDS-PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PDHB Polyclonal Antibody (Product # PA5-27989). Sample (50 µg of whole cell lysate). Lane A: mouse heart. 10% SDS PAGE. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PDHB was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PDHB Polyclonal Antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PDHB Polyclonal Antibody detects Pyruvate Dehydrogenase E1 beta subunit protein at cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PDHB Polyclonal Antibody (Product # PA5-27989) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
