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- Antibody Data
- Antigen structure
- References [38]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 17096-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17096-1-AP, RRID:AB_10598479
- Product name
- SPHK2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SPHK2 antibody (Cat. #17096-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Resveratrol suppresses NSCLC cell growth, invasion and migration by mediating Wnt/β-catenin pathway via downregulating SIX4 and SPHK2.
Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes.
Sphingosine-1-Phosphate Inhibition Increases Endoplasmic Reticulum Stress to Enhance Oxaliplatin Sensitivity in Pancreatic Cancer.
Sphingolipid metabolism controls mammalian heart regeneration.
Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension.
Red Rice Seed Coat Targeting SPHK2 Ameliorated Alcoholic Liver Disease via Restored Intestinal Barrier and Improved Gut Microbiota in Mice.
Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes.
Sphingosine kinase 1/S1P receptor signaling axis is essential for cellular uptake of Neisseria meningitidis in brain endothelial cells.
Sphingosine kinase 2 regulates insulin receptor trafficking in hepatocytes.
Study on the action mechanism of Buyang Huanwu Decoction against ischemic stroke based on S1P/S1PR1/PI3K/Akt signaling pathway.
Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.
Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection.
Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.
Inhibition of Sphingosine Kinase Activity Enhances Immunogenic Cell Surface Exposure of Calreticulin Induced by the Synthetic Cannabinoid 5-epi-CP-55,940.
Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein.
FTY720 Reduces Lipid Accumulation by Upregulating ABCA1 through Liver X Receptor and Sphingosine Kinase 2 Signaling in Macrophages.
The function of Sphingosine-1-phosphate receptor 2 (S1PR2) in maintaining intestinal barrier and inducing ulcerative colitis.
Chronic treatment with serelaxin mitigates adverse remodeling in a murine model of ischemic heart failure and modulates bioactive sphingolipid signaling.
To Be or Not to Be: The Divergent Action and Metabolism of Sphingosine-1 Phosphate in Pancreatic Beta-Cells in Response to Cytokines and Fatty Acids.
Proteomic-Based Approach Reveals the Involvement of Apolipoprotein A-I in Related Phenotypes of Autism Spectrum Disorder in the BTBR Mouse Model.
Sphingosine-1-Phosphate Protects Against the Development of Cardiac Remodeling via Sphingosine Kinase 2 and the S1PR2/ERK Pathway.
Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response.
Inhibition of sphingosine kinase 2 attenuates hypertrophic scar formation via upregulation of Smad7 in human hypertrophic scar fibroblasts.
Analysis of selective target engagement by small-molecule sphingosine kinase inhibitors using the Cellular Thermal Shift Assay (CETSA).
Mechanism of sphingosine 1-phosphate clearance from blood.
Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.
The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.
Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.
Novel TRRAP mutation causes autosomal dominant non-syndromic hearing loss.
Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.
Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.
Flotillin proteins recruit sphingosine to membranes and maintain cellular sphingosine-1-phosphate levels.
Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.
Targeting sphingosine kinase 1 induces MCL1-dependent cell death in acute myeloid leukemia.
Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence.
An oncogenic role for sphingosine kinase 2.
ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.
Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.
Wang X, Liu C, Wang J, Tian Z
Journal of chemotherapy (Florence, Italy) 2024 Sep;36(5):411-421
Journal of chemotherapy (Florence, Italy) 2024 Sep;36(5):411-421
Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes.
Komai M, Noda Y, Ikeda A, Kaneshiro N, Kamikubo Y, Sakurai T, Uehara T, Takasugi N
Journal of lipid research 2024 Mar;65(3):100510
Journal of lipid research 2024 Mar;65(3):100510
Sphingosine-1-Phosphate Inhibition Increases Endoplasmic Reticulum Stress to Enhance Oxaliplatin Sensitivity in Pancreatic Cancer.
Gao Z, Janakiraman H, Xiao Y, Kang SW, Dong J, Choi J, Ogretmen B, Lee HS, Camp ER
World journal of oncology 2024 Apr;15(2):169-180
World journal of oncology 2024 Apr;15(2):169-180
Sphingolipid metabolism controls mammalian heart regeneration.
Ji X, Chen Z, Wang Q, Li B, Wei Y, Li Y, Lin J, Cheng W, Guo Y, Wu S, Mao L, Xiang Y, Lan T, Gu S, Wei M, Zhang JZ, Jiang L, Wang J, Xu J, Cao N
Cell metabolism 2024 Apr 2;36(4):839-856.e8
Cell metabolism 2024 Apr 2;36(4):839-856.e8
Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension.
Ranasinghe ADCU, Holohan M, Borger KM, Donahue DL, Kuc RD, Gerig M, Kim A, Ploplis VA, Castellino FJ, Schwarz MA
Circulation research 2023 Sep 29;133(8):704-719
Circulation research 2023 Sep 29;133(8):704-719
Red Rice Seed Coat Targeting SPHK2 Ameliorated Alcoholic Liver Disease via Restored Intestinal Barrier and Improved Gut Microbiota in Mice.
Chen Y, Zhao Z, Guo S, Li Y, Yin H, Tian L, Cheng G, Li Y
Nutrients 2023 Sep 27;15(19)
Nutrients 2023 Sep 27;15(19)
Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes.
Morishige JI, Yoshioka K, Nakata H, Ishimaru K, Nagata N, Tanaka T, Takuwa Y, Ando H
Journal of lipid research 2023 Nov;64(11):100450
Journal of lipid research 2023 Nov;64(11):100450
Sphingosine kinase 1/S1P receptor signaling axis is essential for cellular uptake of Neisseria meningitidis in brain endothelial cells.
Fohmann I, Weinmann A, Schumacher F, Peters S, Prell A, Weigel C, Spiegel S, Kleuser B, Schubert-Unkmeir A
PLoS pathogens 2023 Nov;19(11):e1011842
PLoS pathogens 2023 Nov;19(11):e1011842
Sphingosine kinase 2 regulates insulin receptor trafficking in hepatocytes.
Aji G, Jiang S, Obulkasim H, Lu Z, Wang W, Xia P
Experimental biology and medicine (Maywood, N.J.) 2023 Jan;248(1):44-51
Experimental biology and medicine (Maywood, N.J.) 2023 Jan;248(1):44-51
Study on the action mechanism of Buyang Huanwu Decoction against ischemic stroke based on S1P/S1PR1/PI3K/Akt signaling pathway.
Liu W, Zhou X, Zeng K, Nie C, Huang J, Zhu L, Pei D, Zhang Y
Journal of ethnopharmacology 2023 Aug 10;312:116471
Journal of ethnopharmacology 2023 Aug 10;312:116471
Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.
Liu JX, Liu M, Yu GZ, Zhao QQ, Wang JL, Sun YH, Koda S, Zhang B, Yu Q, Yan C, Tang RX, Jiang ZH, Zheng KY
Frontiers in cellular and infection microbiology 2022;12:1011378
Frontiers in cellular and infection microbiology 2022;12:1011378
Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection.
Xu M, Xia S, Wang M, Liu X, Li X, Chen W, Wang Y, Li H, Xia C, Chen J, Wu J
PLoS pathogens 2022 Sep;18(9):e1010794
PLoS pathogens 2022 Sep;18(9):e1010794
Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.
Luo L, Gong J, Wang Z, Liu Y, Cao J, Qin J, Zuo R, Zhang H, Wang S, Zhao P, Yang D, Zhang M, Wang Y, Zhang J, Zhou Y, Li C, Ni B, Tian Z, Liu M
Bioactive materials 2022 Sep;15:29-43
Bioactive materials 2022 Sep;15:29-43
Inhibition of Sphingosine Kinase Activity Enhances Immunogenic Cell Surface Exposure of Calreticulin Induced by the Synthetic Cannabinoid 5-epi-CP-55,940.
Hengst JA, Nduwumwami AJ, Raup-Konsavage WM, Vrana KE, Yun JK
Cannabis and cannabinoid research 2022 Oct;7(5):637-647
Cannabis and cannabinoid research 2022 Oct;7(5):637-647
Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein.
Liu XT, Chung LH, Liu D, Chen J, Huang Y, Teo JD, Han XD, Zhao Y, Guan FHX, Tran C, Lee JY, Couttas TA, Liu K, McCaughan GW, Gorrell MD, Don AS, Zhang S, Qi Y
Oncogenesis 2022 Nov 4;11(1):67
Oncogenesis 2022 Nov 4;11(1):67
FTY720 Reduces Lipid Accumulation by Upregulating ABCA1 through Liver X Receptor and Sphingosine Kinase 2 Signaling in Macrophages.
Tachibana K, Kusumoto K, Ogawa M, Ando H, Shimizu T, Ishima Y, Ishida T, Okuhira K
International journal of molecular sciences 2022 Nov 23;23(23)
International journal of molecular sciences 2022 Nov 23;23(23)
The function of Sphingosine-1-phosphate receptor 2 (S1PR2) in maintaining intestinal barrier and inducing ulcerative colitis.
Chen T, Gu K, Lin R, Liu Y, Shan Y
Bioengineered 2022 May;13(5):13703-13717
Bioengineered 2022 May;13(5):13703-13717
Chronic treatment with serelaxin mitigates adverse remodeling in a murine model of ischemic heart failure and modulates bioactive sphingolipid signaling.
Devarakonda T, Valle Raleigh J, Mauro AG, Lambert JM, Cowart LA, Salloum FN
Scientific reports 2022 May 25;12(1):8897
Scientific reports 2022 May 25;12(1):8897
To Be or Not to Be: The Divergent Action and Metabolism of Sphingosine-1 Phosphate in Pancreatic Beta-Cells in Response to Cytokines and Fatty Acids.
Gurgul-Convey E
International journal of molecular sciences 2022 Jan 31;23(3)
International journal of molecular sciences 2022 Jan 31;23(3)
Proteomic-Based Approach Reveals the Involvement of Apolipoprotein A-I in Related Phenotypes of Autism Spectrum Disorder in the BTBR Mouse Model.
Li Q, Shi Y, Li X, Yang Y, Zhang X, Xu L, Ma Z, Wang J, Fan L, Wu L
International journal of molecular sciences 2022 Dec 4;23(23)
International journal of molecular sciences 2022 Dec 4;23(23)
Sphingosine-1-Phosphate Protects Against the Development of Cardiac Remodeling via Sphingosine Kinase 2 and the S1PR2/ERK Pathway.
Yan H, Zhao H, Yi SW, Zhuang H, Wang DW, Jiang JG, Shen GF
Current medical science 2022 Aug;42(4):702-710
Current medical science 2022 Aug;42(4):702-710
Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response.
Vaena S, Chakraborty P, Lee HG, Janneh AH, Kassir MF, Beeson G, Hedley Z, Yalcinkaya A, Sofi MH, Li H, Husby ML, Stahelin RV, Yu XZ, Mehrotra S, Ogretmen B
Cell reports 2021 May 4;35(5):109076
Cell reports 2021 May 4;35(5):109076
Inhibition of sphingosine kinase 2 attenuates hypertrophic scar formation via upregulation of Smad7 in human hypertrophic scar fibroblasts.
Zeng J, Jiang B, Xiao X, Zhang R
Molecular medicine reports 2020 Sep;22(3):2573-2582
Molecular medicine reports 2020 Sep;22(3):2573-2582
Analysis of selective target engagement by small-molecule sphingosine kinase inhibitors using the Cellular Thermal Shift Assay (CETSA).
Hengst JA, Dick TE, Smith CD, Yun JK
Cancer biology & therapy 2020 Sep 1;21(9):841-852
Cancer biology & therapy 2020 Sep 1;21(9):841-852
Mechanism of sphingosine 1-phosphate clearance from blood.
Kharel Y, Huang T, Salamon A, Harris TE, Santos WL, Lynch KR
The Biochemical journal 2020 Mar 13;477(5):925-935
The Biochemical journal 2020 Mar 13;477(5):925-935
Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.
Blais-Lecours P, Laouafa S, Arias-Reyes C, Santos WL, Joseph V, Burgess JK, Halayko AJ, Soliz J, Marsolais D
American journal of respiratory cell and molecular biology 2020 Jan;62(1):35-42
American journal of respiratory cell and molecular biology 2020 Jan;62(1):35-42
The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.
Li J, Zhao LM, Zhang C, Li M, Gao B, Hu XH, Cao J, Wang GY
Oncology research 2020 Feb 7;28(1):51-63
Oncology research 2020 Feb 7;28(1):51-63
Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.
Yu J, Dong J, Chen K, Ding Y, Yang Z, Lan T
Transgenic research 2020 Aug;29(4):419-428
Transgenic research 2020 Aug;29(4):419-428
Novel TRRAP mutation causes autosomal dominant non-syndromic hearing loss.
Xia W, Hu J, Ma J, Huang J, Wang X, Jiang N, Zhang J, Ma Z, Ma D
Clinical genetics 2019 Oct;96(4):300-308
Clinical genetics 2019 Oct;96(4):300-308
Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.
Xiao J, Lin H, Liu B, Xia Z, Zhang J, Jin J
Biomarkers in medicine 2019 Jun;13(8):627-637
Biomarkers in medicine 2019 Jun;13(8):627-637
Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.
Neubauer HA, Tea MN, Zebol JR, Gliddon BL, Stefanidis C, Moretti PAB, Pitman MR, Costabile M, Kular J, Stringer BW, Day BW, Samuel MS, Bonder CS, Powell JA, Pitson SM
Oncogene 2019 Feb;38(8):1151-1165
Oncogene 2019 Feb;38(8):1151-1165
Flotillin proteins recruit sphingosine to membranes and maintain cellular sphingosine-1-phosphate levels.
Riento K, Zhang Q, Clark J, Begum F, Stephens E, Wakelam MJ, Nichols BJ
PloS one 2018;13(5):e0197401
PloS one 2018;13(5):e0197401
Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.
Panneer Selvam S, Roth BM, Nganga R, Kim J, Cooley MA, Helke K, Smith CD, Ogretmen B
The Journal of biological chemistry 2018 Jun 22;293(25):9784-9800
The Journal of biological chemistry 2018 Jun 22;293(25):9784-9800
Targeting sphingosine kinase 1 induces MCL1-dependent cell death in acute myeloid leukemia.
Powell JA, Lewis AC, Zhu W, Toubia J, Pitman MR, Wallington-Beddoe CT, Moretti PA, Iarossi D, Samaraweera SE, Cummings N, Ramshaw HS, Thomas D, Wei AH, Lopez AF, D'Andrea RJ, Lewis ID, Pitson SM
Blood 2017 Feb 9;129(6):771-782
Blood 2017 Feb 9;129(6):771-782
Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence.
Neubauer HA, Pitson SM
F1000Research 2016;5:2825
F1000Research 2016;5:2825
An oncogenic role for sphingosine kinase 2.
Neubauer HA, Pham DH, Zebol JR, Moretti PA, Peterson AL, Leclercq TM, Chan H, Powell JA, Pitman MR, Samuel MS, Bonder CS, Creek DJ, Gliddon BL, Pitson SM
Oncotarget 2016 Oct 4;7(40):64886-64899
Oncotarget 2016 Oct 4;7(40):64886-64899
ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.
Liu X, Ren K, Suo R, Xiong SL, Zhang QH, Mo ZC, Tang ZL, Jiang Y, Peng XS, Yi GH
Journal of physiology and biochemistry 2016 Dec;72(4):657-667
Journal of physiology and biochemistry 2016 Dec;72(4):657-667
Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.
Sanchez T, Estrada-Hernandez T, Paik JH, Wu MT, Venkataraman K, Brinkmann V, Claffey K, Hla T
The Journal of biological chemistry 2003 Nov 21;278(47):47281-90
The Journal of biological chemistry 2003 Nov 21;278(47):47281-90
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17096-1-AP(SPHK2 antibody) at dilution of 1:300
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 17096-1-AP(SPHK2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPHK2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPHK2. This antibody recognizes human,mouse,rat antigen. The SPHK2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
