17096-1-AP

antibody from Proteintech Group

Targeting: SPHK2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17096-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#17096-1-AP, RRID:AB_10598479

Product name

SPHK2 antibody

Antibody type

Polyclonal

Description

KD/KO validated SPHK2 antibody (Cat. #17096-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Noncanonical function of sphingosine kinase 2 (SPHK2) sustains hepatic triglyceride homeostasis.

Yuan B, Ding X, Deng X, Wu T, Xu N, Wang Y, Yang Y, Wang W, Ren X, Cheng W, Li R, Zhao H, Guo J, Li J, Dong W, Zhang Y, Lan T
British journal of pharmacology 2026 Jun;183(12):3272-3292

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SPHK2 inhibition alleviates chronic intermittent hypoxia-induced inflammation in adipose tissue by decreasing endoplasmic reticulum stress.

Ran L, Wei C, Wang X, Zhao Y, Li P, Huang D, Gu W, Wu X, Liang Z, Wang X, Gong L
European journal of pharmacology 2025 Sep 5;1002:177841

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PLK1-mediated phosphorylation of PHGDH reprograms serine metabolism in advanced prostate cancer.

Rao X, Allison DB, Flight RM, Lin P, He D, Li Z, Zhang Y, Wang R, Li C, Wang J, Wang X, Peng J, Fong KW, Shao Q, Wang C, Ali ES, Moseley HN, Liu X
bioRxiv : the preprint server for biology 2025 May 24;

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S1P/S1PRs-TRPV4 axis is a novel therapeutic target for persistent pain and itch in chronic dermatitis.

Zhang X, Zhou Y, Wang C, Ren J, Wang Y, Liu P, Feng W, Li X, Qi M, Yang Y, Zhu C, Wang F, Ma Y, Tang Z, Yu G
British journal of pharmacology 2025 Mar;182(5):1223-1235

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Elevated Sphingosine Levels Suppress Profibrotic TGF-β Signaling via the PKC/miR-21/SMAD7 Axis in Sphingosine Kinase 2-Deficient Renal Fibroblasts and Unilateral Ureteral Obstruction-Induced Kidney Fibrosis.

Oftring A, Wagner V, Thomas D, Huber LS, Geisslinger G, Schaefer L, Vutukuri R, Pfeilschifter J, Schwalm S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jun 30;39(12):e70768

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Site- and cell-type-specific miRNA and mRNA genes and networks across the cortex, striatum, and hypothalamus.

Zacharias AM, O'Connor CD, Topouza DG, Fang ZY, Ghazinejad H, Chen H, Duan Q, Ghasemlou N
Communications biology 2025 Jul 1;8(1):969

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Hypoxia-inducible factor 2α overexpression in podocytes ameliorates lipid metabolism disorders in diabetic kidney disease by inhibiting S1P.

Yang X, Wang Q, Nie H, Wang M, Wang Y, Li S, Liu Q, Liu G
Renal failure 2025 Dec;47(1):2568972

---

Inhibition of advanced glycation end-products by the vitamin B6 vitamer pyridoxamine prevents systemic and skeletal muscle diet-induced metaflammation through modulation of S1P/RhoA/ROCK signalling.

Rivas Navarrete PI, Collotta D, Dal Bello F, Cento AS, Aragno M, Collino M, Mastrocola R
The Journal of nutritional biochemistry 2025 Dec;146:110068

---

Sanleng Wan inhibits endometriosis progression by regulating sphingolipid metabolism via the S1P/S1PR1-Akt axis based on serum metabolomics and network pharmacology.

Fu Z, Zhou H, Liu H, Li X, Cao L, Zheng W
Journal of ethnopharmacology 2025 Aug 29;352:120153

---

Hydroxyl safflower yellow A regulates bone-fat balance in osteoporosis by SphK1/S1P/S1PR signaling pathway.

Xu X, Hu X, Zhou S, Liu J, Su Z, Zheng Z, Luo D
Biochemical pharmacology 2025 Aug 20;242(Pt 1):117258

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Resveratrol suppresses NSCLC cell growth, invasion and migration by mediating Wnt/β-catenin pathway via downregulating SIX4 and SPHK2.

Wang X, Liu C, Wang J, Tian Z
Journal of chemotherapy (Florence, Italy) 2024 Sep;36(5):411-421

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Targeting SNAI1-Mediated Colorectal Cancer Chemoresistance and Stemness by Sphingosine Kinase 2 Inhibition.

Janakiraman H, Gao Z, Zhu Y, Dong J, Becker SA, Janneh A, Ogretmen B, Camp ER
World journal of oncology 2024 Oct;15(5):744-757

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An inducible sphingosine kinase 1 in hepatic stellate cells potentiates liver fibrosis.

Baek JS, Lee JH, Kim JH, Cho SS, Kim YS, Yang JH, Shin EJ, Kang HG, Kim SJ, Ahn SG, Park EY, Baek DJ, Yim SK, Kang KW, Ki SH, Kim KM
Biochemical pharmacology 2024 Nov;229:116520

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Hydroxysafflor yellow A, a natural food pigment, ameliorates atherosclerosis in ApoE(-/-) mice by inhibiting the SphK1/S1P/S1PR3 pathway.

Liu Y, Piao J, He J, Su Z, Luo Z, Luo D
Food science & nutrition 2024 Nov;12(11):8939-8955

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The Species Effect: Differential Sphingosine-1-Phosphate Responses in the Bone in Human Versus Mouse.

Frost K, Lewis JW, Jones SW, Edwards JR, Naylor AJ, McGettrick HM
International journal of molecular sciences 2024 May 8;25(10)

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Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes.

Komai M, Noda Y, Ikeda A, Kaneshiro N, Kamikubo Y, Sakurai T, Uehara T, Takasugi N
Journal of lipid research 2024 Mar;65(3):100510

---

Lathyrol reduces the RCC invasion and incidence of EMT via affecting the expression of AR and SPHK2 in RCC mice.

Song S, Tai L, Xu Y, Jiang J, Zhou L, Zhao J
Discover oncology 2024 Jul 4;15(1):264

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A role for plasma membrane Ca(2+) ATPases in regulation of cellular Ca(2+) homeostasis by sphingosine kinase-1.

Volk LM, Bruun JE, Trautmann S, Thomas D, Schwalm S, Pfeilschifter J, Zu Heringdorf DM
Pflugers Archiv : European journal of physiology 2024 Dec;476(12):1895-1911

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A multi-omics approach identifies the key role of disorders of sphingolipid metabolism in Ang II-induced hypertensive cardiomyopathy myocardial remodeling.

Qu Y, Ma D, Wu T, Wang H, Tian Z, Liu X, Wang Y
Scientific reports 2024 Dec 5;14(1):30379

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5-Fluorouracil resistance due to sphingosine kinase 2 overexpression in colorectal cancer is associated with myeloid-derived suppressor cell-mediated immunosuppressive effects.

Li X, Chen Y, Liang Y, Shi W
BMC cancer 2024 Aug 8;24(1):983

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Sphingosine-1-Phosphate Inhibition Increases Endoplasmic Reticulum Stress to Enhance Oxaliplatin Sensitivity in Pancreatic Cancer.

Gao Z, Janakiraman H, Xiao Y, Kang SW, Dong J, Choi J, Ogretmen B, Lee HS, Camp ER
World journal of oncology 2024 Apr;15(2):169-180

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Sphingolipid metabolism controls mammalian heart regeneration.

Ji X, Chen Z, Wang Q, Li B, Wei Y, Li Y, Lin J, Cheng W, Guo Y, Wu S, Mao L, Xiang Y, Lan T, Gu S, Wei M, Zhang JZ, Jiang L, Wang J, Xu J, Cao N
Cell metabolism 2024 Apr 2;36(4):839-856.e8

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Altered Smooth Muscle Cell Histone Acetylome by the SPHK2/S1P Axis Promotes Pulmonary Hypertension.

Ranasinghe ADCU, Holohan M, Borger KM, Donahue DL, Kuc RD, Gerig M, Kim A, Ploplis VA, Castellino FJ, Schwarz MA
Circulation research 2023 Sep 29;133(8):704-719

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Red Rice Seed Coat Targeting SPHK2 Ameliorated Alcoholic Liver Disease via Restored Intestinal Barrier and Improved Gut Microbiota in Mice.

Chen Y, Zhao Z, Guo S, Li Y, Yin H, Tian L, Cheng G, Li Y
Nutrients 2023 Sep 27;15(19)

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Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes.

Morishige JI, Yoshioka K, Nakata H, Ishimaru K, Nagata N, Tanaka T, Takuwa Y, Ando H
Journal of lipid research 2023 Nov;64(11):100450

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Sphingosine kinase 1/S1P receptor signaling axis is essential for cellular uptake of Neisseria meningitidis in brain endothelial cells.

Fohmann I, Weinmann A, Schumacher F, Peters S, Prell A, Weigel C, Spiegel S, Kleuser B, Schubert-Unkmeir A
PLoS pathogens 2023 Nov;19(11):e1011842

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Sphingosine kinase 2 regulates insulin receptor trafficking in hepatocytes.

Aji G, Jiang S, Obulkasim H, Lu Z, Wang W, Xia P
Experimental biology and medicine (Maywood, N.J.) 2023 Jan;248(1):44-51

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Study on the action mechanism of Buyang Huanwu Decoction against ischemic stroke based on S1P/S1PR1/PI3K/Akt signaling pathway.

Liu W, Zhou X, Zeng K, Nie C, Huang J, Zhu L, Pei D, Zhang Y
Journal of ethnopharmacology 2023 Aug 10;312:116471

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Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2.

Liu JX, Liu M, Yu GZ, Zhao QQ, Wang JL, Sun YH, Koda S, Zhang B, Yu Q, Yan C, Tang RX, Jiang ZH, Zheng KY
Frontiers in cellular and infection microbiology 2022;12:1011378

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Enzymatic independent role of sphingosine kinase 2 in regulating the expression of type I interferon during influenza A virus infection.

Xu M, Xia S, Wang M, Liu X, Li X, Chen W, Wang Y, Li H, Xia C, Chen J, Wu J
PLoS pathogens 2022 Sep;18(9):e1010794

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Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Luo L, Gong J, Wang Z, Liu Y, Cao J, Qin J, Zuo R, Zhang H, Wang S, Zhao P, Yang D, Zhang M, Wang Y, Zhang J, Zhou Y, Li C, Ni B, Tian Z, Liu M
Bioactive materials 2022 Sep;15:29-43

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Inhibition of Sphingosine Kinase Activity Enhances Immunogenic Cell Surface Exposure of Calreticulin Induced by the Synthetic Cannabinoid 5-epi-CP-55,940.

Hengst JA, Nduwumwami AJ, Raup-Konsavage WM, Vrana KE, Yun JK
Cannabis and cannabinoid research 2022 Oct;7(5):637-647

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Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein.

Liu XT, Chung LH, Liu D, Chen J, Huang Y, Teo JD, Han XD, Zhao Y, Guan FHX, Tran C, Lee JY, Couttas TA, Liu K, McCaughan GW, Gorrell MD, Don AS, Zhang S, Qi Y
Oncogenesis 2022 Nov 4;11(1):67

---

FTY720 Reduces Lipid Accumulation by Upregulating ABCA1 through Liver X Receptor and Sphingosine Kinase 2 Signaling in Macrophages.

Tachibana K, Kusumoto K, Ogawa M, Ando H, Shimizu T, Ishima Y, Ishida T, Okuhira K
International journal of molecular sciences 2022 Nov 23;23(23)

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The function of Sphingosine-1-phosphate receptor 2 (S1PR2) in maintaining intestinal barrier and inducing ulcerative colitis.

Chen T, Gu K, Lin R, Liu Y, Shan Y
Bioengineered 2022 May;13(5):13703-13717

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Chronic treatment with serelaxin mitigates adverse remodeling in a murine model of ischemic heart failure and modulates bioactive sphingolipid signaling.

Devarakonda T, Valle Raleigh J, Mauro AG, Lambert JM, Cowart LA, Salloum FN
Scientific reports 2022 May 25;12(1):8897

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To Be or Not to Be: The Divergent Action and Metabolism of Sphingosine-1 Phosphate in Pancreatic Beta-Cells in Response to Cytokines and Fatty Acids.

Gurgul-Convey E
International journal of molecular sciences 2022 Jan 31;23(3)

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Proteomic-Based Approach Reveals the Involvement of Apolipoprotein A-I in Related Phenotypes of Autism Spectrum Disorder in the BTBR Mouse Model.

Li Q, Shi Y, Li X, Yang Y, Zhang X, Xu L, Ma Z, Wang J, Fan L, Wu L
International journal of molecular sciences 2022 Dec 4;23(23)

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Sphingosine-1-Phosphate Protects Against the Development of Cardiac Remodeling via Sphingosine Kinase 2 and the S1PR2/ERK Pathway.

Yan H, Zhao H, Yi SW, Zhuang H, Wang DW, Jiang JG, Shen GF
Current medical science 2022 Aug;42(4):702-710

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Aging-dependent mitochondrial dysfunction mediated by ceramide signaling inhibits antitumor T cell response.

Vaena S, Chakraborty P, Lee HG, Janneh AH, Kassir MF, Beeson G, Hedley Z, Yalcinkaya A, Sofi MH, Li H, Husby ML, Stahelin RV, Yu XZ, Mehrotra S, Ogretmen B
Cell reports 2021 May 4;35(5):109076

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Inhibition of sphingosine kinase 2 attenuates hypertrophic scar formation via upregulation of Smad7 in human hypertrophic scar fibroblasts.

Zeng J, Jiang B, Xiao X, Zhang R
Molecular medicine reports 2020 Sep;22(3):2573-2582

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Analysis of selective target engagement by small-molecule sphingosine kinase inhibitors using the Cellular Thermal Shift Assay (CETSA).

Hengst JA, Dick TE, Smith CD, Yun JK
Cancer biology & therapy 2020 Sep 1;21(9):841-852

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Mechanism of sphingosine 1-phosphate clearance from blood.

Kharel Y, Huang T, Salamon A, Harris TE, Santos WL, Lynch KR
The Biochemical journal 2020 Mar 13;477(5):925-935

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Metabolic Adaptation of Airway Smooth Muscle Cells to an SPHK2 Substrate Precedes Cytostasis.

Blais-Lecours P, Laouafa S, Arias-Reyes C, Santos WL, Joseph V, Burgess JK, Halayko AJ, Soliz J, Marsolais D
American journal of respiratory cell and molecular biology 2020 Jan;62(1):35-42

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The lncRNA FEZF1-AS1 Promotes the Progression of Colorectal Cancer Through Regulating OTX1 and Targeting miR-30a-5p.

Li J, Zhao LM, Zhang C, Li M, Gao B, Hu XH, Cao J, Wang GY
Oncology research 2020 Feb 7;28(1):51-63

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Generation of mice with hepatocyte-specific conditional deletion of sphingosine kinase 1.

Yu J, Dong J, Chen K, Ding Y, Yang Z, Lan T
Transgenic research 2020 Aug;29(4):419-428

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Novel TRRAP mutation causes autosomal dominant non-syndromic hearing loss.

Xia W, Hu J, Ma J, Huang J, Wang X, Jiang N, Zhang J, Ma Z, Ma D
Clinical genetics 2019 Oct;96(4):300-308

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Decreased S1P and SPHK2 are involved in pancreatic acinar cell injury.

Xiao J, Lin H, Liu B, Xia Z, Zhang J, Jin J
Biomarkers in medicine 2019 Jun;13(8):627-637

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Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma.

Neubauer HA, Tea MN, Zebol JR, Gliddon BL, Stefanidis C, Moretti PAB, Pitman MR, Costabile M, Kular J, Stringer BW, Day BW, Samuel MS, Bonder CS, Powell JA, Pitson SM
Oncogene 2019 Feb;38(8):1151-1165

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The role of sphingosine kinase 2 in alcoholic liver disease.

Kwong EK, Liu R, Zhao D, Li X, Zhu W, Wang X, Gurley EC, Lai G, Liu J, Hylemon PB, Zhou H
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2019 Aug;51(8):1154-1163

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Flotillin proteins recruit sphingosine to membranes and maintain cellular sphingosine-1-phosphate levels.

Riento K, Zhang Q, Clark J, Begum F, Stephens E, Wakelam MJ, Nichols BJ
PloS one 2018;13(5):e0197401

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Balance between senescence and apoptosis is regulated by telomere damage-induced association between p16 and caspase-3.

Panneer Selvam S, Roth BM, Nganga R, Kim J, Cooley MA, Helke K, Smith CD, Ogretmen B
The Journal of biological chemistry 2018 Jun 22;293(25):9784-9800

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Targeting sphingosine kinase 1 induces MCL1-dependent cell death in acute myeloid leukemia.

Powell JA, Lewis AC, Zhu W, Toubia J, Pitman MR, Wallington-Beddoe CT, Moretti PA, Iarossi D, Samaraweera SE, Cummings N, Ramshaw HS, Thomas D, Wei AH, Lopez AF, D'Andrea RJ, Lewis ID, Pitson SM
Blood 2017 Feb 9;129(6):771-782

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Validation of commercially available sphingosine kinase 2 antibodies for use in immunoblotting, immunoprecipitation and immunofluorescence.

Neubauer HA, Pitson SM
F1000Research 2016;5:2825

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An oncogenic role for sphingosine kinase 2.

Neubauer HA, Pham DH, Zebol JR, Moretti PA, Peterson AL, Leclercq TM, Chan H, Powell JA, Pitman MR, Samuel MS, Bonder CS, Creek DJ, Gliddon BL, Pitson SM
Oncotarget 2016 Oct 4;7(40):64886-64899

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ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.

Liu X, Ren K, Suo R, Xiong SL, Zhang QH, Mo ZC, Tang ZL, Jiang Y, Peng XS, Yi GH
Journal of physiology and biochemistry 2016 Dec;72(4):657-667

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Phosphorylation and action of the immunomodulator FTY720 inhibits vascular endothelial cell growth factor-induced vascular permeability.

Sanchez T, Estrada-Hernandez T, Paik JH, Wu MT, Venkataraman K, Brinkmann V, Claffey K, Hla T
The Journal of biological chemistry 2003 Nov 21;278(47):47281-90

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17096-1-AP(SPHK2 antibody) at dilution of 1:300

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 17096-1-AP(SPHK2 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The SPHK2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPHK2. This antibody recognizes human,mouse,rat antigen. The SPHK2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

17096-1-AP.pdf

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