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Western blotAntibody data
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- Antigen structure
- References [5]
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- Western blot [2]
- Immunocytochemistry [3]
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- Product number
- GTX101241 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101241, RRID:AB_1950186
- Product name
- eIF2 alpha antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Fucoidan induces Toll-like receptor 4-regulated reactive oxygen species and promotes endoplasmic reticulum stress-mediated apoptosis in lung cancer.
Fisetin Protects PC12 Cells from Tunicamycin-Mediated Cell Death via Reactive Oxygen Species Scavenging and Modulation of Nrf2-Driven Gene Expression, SIRT1 and MAPK Signaling in PC12 Cells.
Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Lipid peroxidation end product 4-hydroxy-trans-2-nonenal triggers unfolded protein response and heme oxygenase-1 expression in PC12 cells: Roles of ROS and MAPK pathways.
Hsu HY, Lin TY, Lu MK, Leng PJ, Tsao SM, Wu YC
Scientific reports 2017 Mar 23;7:44990
Scientific reports 2017 Mar 23;7:44990
Fisetin Protects PC12 Cells from Tunicamycin-Mediated Cell Death via Reactive Oxygen Species Scavenging and Modulation of Nrf2-Driven Gene Expression, SIRT1 and MAPK Signaling in PC12 Cells.
Yen JH, Wu PS, Chen SF, Wu MJ
International journal of molecular sciences 2017 Apr 17;18(4)
International journal of molecular sciences 2017 Apr 17;18(4)
Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
Wu PS, Yen JH, Kou MC, Wu MJ
PloS one 2015;10(6):e0130599
PloS one 2015;10(6):e0130599
A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Lee KH, Lo HL, Tang WC, Hsiao HH, Yang PM
Scientific reports 2014 Sep 17;4:6394
Scientific reports 2014 Sep 17;4:6394
Lipid peroxidation end product 4-hydroxy-trans-2-nonenal triggers unfolded protein response and heme oxygenase-1 expression in PC12 cells: Roles of ROS and MAPK pathways.
Lin MH, Yen JH, Weng CY, Wang L, Ha CL, Wu MJ
Toxicology 2014 Jan 6;315:24-37
Toxicology 2014 Jan 6;315:24-37
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:H129912% SDS PAGEGTX101241 diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using eIF2 alpha(GTX101241) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:5000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
