Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF3997 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal eIF2 alpha/EIF2S1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat eIF2 alpha in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Silvestrol Inhibits Chikungunya Virus Replication.
Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection.
Henss L, Scholz T, Grünweller A, Schnierle BS
Viruses 2018 Oct 30;10(11)
Viruses 2018 Oct 30;10(11)
Engineered mRNA-expressed antibodies prevent respiratory syncytial virus infection.
Tiwari PM, Vanover D, Lindsay KE, Bawage SS, Kirschman JL, Bhosle S, Lifland AW, Zurla C, Santangelo PJ
Nature communications 2018 Oct 1;9(1):3999
Nature communications 2018 Oct 1;9(1):3999
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human eIF2 alpha by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF2 alpha at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human eIF2 alpha by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for eIF2 alpha at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eIF2 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3997) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.