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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00001965-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001965-M01, RRID:AB_538494
- Product name
- Mouse Monoclonal eIF2 alpha/EIF2S1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EIF2S1 - eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR.
Weinlich S, Hüttelmaier S, Schierhorn A, Behrens SE, Ostareck-Lederer A, Ostareck DH
RNA (New York, N.Y.) 2009 Aug;15(8):1528-42
RNA (New York, N.Y.) 2009 Aug;15(8):1528-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi ( Cat # H00001965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody (M01), clone 3H12-C11 (Cat # H00001965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody (M01), clone 3H12-C11.Lane 1: EIF2S1 transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - EIF2S1 monoclonal antibody (M01), clone 3H12-C11. Analysis of EIF2S1 expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - EIF2S1 monoclonal antibody (M01), clone 3H12-C11 Analysis of EIF2S1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Detection limit for recombinant GST tagged EIF2S1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of monoclonal antibody to EIF2S1 on HeLa cell. Antibody concentration 10 ug/ml.
