TA501314

antibody from OriGene

Targeting: EIF2S1 EIF-2alpha, EIF2, EIF2A

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501314

Provider

OriGene

Proper citation

OriGene Cat#TA501314, RRID:AB_11125687

Product name

Anti-EIF2S1 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)

Antibody type

Monoclonal

Description

Anti-EIF2S1 mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

EIF2S1

Isotype

IgG

Antibody clone number

OTI3H7

Vial size

100 µl

Concentration

0.62 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200368 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF2S1 antibody(TA501314), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody(TA501314),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC