TA501373

antibody from OriGene

Targeting: EIF2S1 EIF-2alpha, EIF2, EIF2A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501373

Provider

OriGene

Proper citation

OriGene Cat#TA501373, RRID:AB_11124035

Product name

Anti-EIF2S1 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Antibody type

Monoclonal

Description

Anti-EIF2S1 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

EIF2S1

Isotype

IgG

Antibody clone number

OTI1B10

Vial size

100 µl

Concentration

0.58 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501373, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501373, Dilution 1:50)

Validation comment

IHC