- MA5-15133 - Invitrogen Antibodies EIF2S1 antibody

Submitted references Host Tau Genotype Specifically Designs and Regulates Tau Seeding and Spreading and Host Tau Transformation Following Intrahippocampal Injection of Identical Tau AD Inoculum.

Andrés-Benito P, Carmona M, Jordán M, Fernández-Irigoyen J, Santamaría E, Del Rio JA, Ferrer I
International journal of molecular sciences 2022 Jan 10;23(2)

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Karunarathne WAHM, Molagoda IMN, Lee KT, Choi YH, Yu SM, Kang CH, Kim GY
Antioxidants (Basel, Switzerland) 2021 Apr 9;10(4)

IRE1A Stimulates Hepatocyte-Derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis.

Dasgupta D, Nakao Y, Mauer AS, Thompson JM, Sehrawat TS, Liao CY, Krishnan A, Lucien F, Guo Q, Liu M, Xue F, Fukushima M, Katsumi T, Bansal A, Pandey MK, Maiers JL, DeGrado T, Ibrahim SH, Revzin A, Pavelko KD, Barry MA, Kaufman RJ, Malhi H
Gastroenterology 2020 Oct;159(4):1487-1503.e17

Mytych J, Sołek P, Będzińska A, Rusinek K, Warzybok A, Tabęcka-Łonczyńska A, Koziorowski M
Cells 2020 Jan 21;9(2)

Extracellular and ER-stored Ca(2+) contribute to BIRD-2-induced cell death in diffuse large B-cell lymphoma cells.

Bittremieux M, La Rovere RM, Schuermans M, Luyten T, Mikoshiba K, Vangheluwe P, Parys JB, Bultynck G
Cell death discovery 2018;4:101

Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.

Frau-Méndez MA, Fernández-Vega I, Ansoleaga B, Blanco Tech R, Carmona Tech M, Antonio Del Rio J, Zerr I, Llorens F, José Zarranz J, Ferrer I
Brain pathology (Zurich, Switzerland) 2017 Jan;27(1):95-106

RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response.

Green KM, Glineburg MR, Kearse MG, Flores BN, Linsalata AE, Fedak SJ, Goldstrohm AC, Barmada SJ, Todd PK
Nature communications 2017 Dec 8;8(1):2005

The High-Risk Human Papillomavirus E6 Oncogene Exacerbates the Negative Effect of Tryptophan Starvation on the Development of Chlamydia trachomatis.

Sherchand SP, Ibana JA, Zea AH, Quayle AJ, Aiyar A
PloS one 2016;11(9):e0163174

VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation.

Shih YT, Hsueh YP
Nature communications 2016 Mar 17;7:11020

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Phospho-eIF2-alpha pSer52 in extracts from HeLa cells, untreated or phosphatase-treated, using Phospho-eIF2-alpha pSer52 monoclonal antibody (Product # MA5-15133).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of Phospho-eIF2-alpha pSer52 in paraffin-embedded human lung carcinoma using a Phospho-eIF2-alpha pSer52 monoclonal antibody (Product # MA5-15133) in the presence of control peptide (left) or Phospho-eIF2alpha (Ser52) blocking peptide (right).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 7 BIRD-2-triggered apoptosis is reduced by depleting the ER Ca 2+ store in SU-DHL-4 cells. a Analysis of the cytosolic Ca 2+ response in SU-DHL-4 cells using Fura-2 AM. After the addition of 3 mM EGTA, 1 or 10 uM TG or 10 uM CPA were added to deplete the ER Ca 2+ store. The curves represent the mean +- SEM of three independent experiments. The TG/CPA-releasable Ca 2+ is quantified by measuring the AUC ( F 340 / F 380 x s), which is shown in b . c Representative scatter plots from flow cytometry analysis detecting apoptosis in SU-DHL-4 cells stained with Annexin V-FITC and 7-AAD. Cells were pre-treated with or without 1 uM TG 30 min prior to application of 10 uM BIRD-2. After 2 h of BIRD-2 treatment, apoptotic cell death was detected by measuring the Annexin V-FITC-positive fraction. d Quantitative analysis of five independent experiments detecting apoptosis in SU-DHL-4 cells treated for 2 h with 10 uM BIRD-2, with or without a pre-treatment of 30 min with 1 uM TG. e Quantitative analysis of five independent experiments detecting apoptosis in SU-DHL-4 cells treated for 2 h with 10 uM BIRD-2, with or without a pre-treatment of 30 min with 10 uM CPA. f A representative western blot of four independent experiments showing the expression levels of total eIF2alpha and p-eIF2alpha in SU-DHL-4 cells treated for 4 h with 5ug/ml tunicamycin. The expression level of vinculin was used as a loading control. g Quantification of the p-eIF2alpha/total eIF2alpha-protein

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of immunoprecipitates from PC12 cells (Lane 1) lysate control (Lane 2) antibody alone as negative control (Lane 3) antibody immunocomplex of PC12 cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig 2 Temporal evaluation of eIF2alpha phosphorylation in HeLa and C33A grown in tryptophan-free media. HeLa and C33A cells were plated in complete media, which was replaced with tryptophan-free media 24 hours post-plating. Cells were harvested every 6 hours and used to make extracts that were evaluated by immunoblot using antibodies against eIF2alpha or eIF2alpha phosphorylated on serine 51 (p-eIF2alpha). Immunoblots against beta-actin were performed as a loading control. Similar results were obtained from three independent experiments.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 8 Effect of anthocyanins from the flower petals of Hibiscus syriacus L. (Malvaceae, AHs) on ER stress and mtROS production. ( A ) and ( B ) The expression of GRP78, ATF4, p-eIF1alpha, CHOP, and beta-actin protein (left) and relative density (right). ( C ) The staining of MitoSOX Red and MitoTracker Green. ( D ) Survival rate of zebrafish. * p < 0.05 and *** p < 0.001 vs. UVB-irradiated cells and ### p < 0.001 vs. untreated cells (UT).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 RAN translation is selectively activated by the integrated stress response. a Schematic of the integrated stress response pathway. b Western blot analysis of the ER stress pathway and C9RAN reporter levels in HEK293 cells after treatment with 2 muM TG. GAPDH was used as a loading control. c Expression of control and C9RAN NLuc reporters and co-transfected FLuc in HEK293 cells treated with 2 muM TG, n = 9. d Schematic of the previously published 36 +1 and +2 CGG RAN translation NLuc reporters. e , f Expression of control and CGG RAN translation reporters and co-transfected FLuc in HEK293T cells treated with 2 muM TG analyzed by ( e ) luciferase activity, n = 9, and ( f ) anti-FLAG western blot. Tubulin was used as a loading control. g Fluorescence intensity of mApple and co-transfected GFP (left) or (G 4 C 2 )x66-GFP (right) in primary rat cortical neurons, imaged with automated fluorescent microscopy 3 days after treatment with 0.5, 1, or 2 muM TG, n > 30. Graphs represent mean +- SEM. Two-tailed Student's t test with Bonferroni and Welch's correction, ** p < 0.01; *** p < 0.001; **** p < 0.0001. PERK endoplasmic reticulum ER-resident kinase, HRI heme-regulated inhibitor kinase, SA sodium arsenite, TG thapsigargin, TM tunicamycin

MA5-15133

Antibody data