NBP2-02669

antibody from Novus Biologicals

Targeting: EIF2S1 EIF-2alpha, EIF2, EIF2A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-02669

Provider

Novus Biologicals

Product name

Mouse Monoclonal eIF2 alpha/EIF2S1 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eIF2A.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-eIF2A mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-eIF2A mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human Kidney tissue using anti-eIF2A mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2A mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2A mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: EIF2S1 Antibody (3H4) [NBP2-02669] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-eIF2A antibody, and then analyzed by flow cytometry.