Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BML-SA405-0020 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA405-0020, RRID:AB_2050517
- Product name
- [pSer52]Eukaryotic Initiation factor 2 _ polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Yeast
- Host
- Rabbit
- Vial size
- 20 μl
- Storage
- -80°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80¡C.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Mouse 3T3 cell extracts were blotted and incubated with anti-eIF2-_ (pS52) antibody following (1) prior incubation in the presence of the peptide immunogen, (2) the nonphosphopeptide corresponding to the eIF2-_ (pS51) phosphopeptide, (3) or no peptide. Only the phospho-peptide corresponding to the region surrounding serine 51 (serine 52 in mouse) blocks the antibody signal.