Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- BAF3138 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Granulysin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Granulysin in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human NKG-2D is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Granulysin in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were treated for 24 hr with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin, then stained with Goat Anti-Human Granulysin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3138, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).