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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Flow cytometry [2]
- Other assay [4]
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- Product number
- 12-8828-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Granulysin Monoclonal Antibody (eBioDH2 (DH2)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin is a member of the saposin-like protein (SAPLIP) family. Two proteins 9 and 15kDa are thought to be post translation modified versions of the 15kDa protein with a change in confirmation. Granulysin is found in NK and activated T cells and the expression is confined to the cytolytic granules which also contains perforin and granzyme. Granulysin with perforn are suspected to function synergistically to kill bacteria and induce apoptosis in nucleated cells.
- Conjugate
- Yellow dye
- Antibody clone number
- eBioDH2 (DH2)
- Concentration
- 5 µL/Test
Submitted references Nanocages engineered from Bacillus Calmette-Guerin facilitate protective Vγ2Vδ2 T cell immunity against Mycobacterium tuberculosis infection.
Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.
Human newborn bacille Calmette-Guérin vaccination and risk of tuberculosis disease: a case-control study.
Human decidual tissue contains differentiated CD8+ effector-memory T cells with unique properties.
Only a subset of phosphoantigen-responsive gamma9delta2 T cells mediate protective tuberculosis immunity.
Human NKT cells express granulysin and exhibit antimycobacterial activity.
T-cell release of granulysin contributes to host defense in leprosy.
Biosynthesis of granulysin, a novel cytolytic molecule.
Pi J, Zhang Z, Yang E, Chen L, Zeng L, Chen Y, Wang R, Huang D, Fan S, Lin W, Shen H, Xu JF, Zeng G, Shen L
Journal of nanobiotechnology 2022 Jan 15;20(1):36
Journal of nanobiotechnology 2022 Jan 15;20(1):36
Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.
Kathamuthu GR, Kumar NP, Moideen K, Menon PA, Babu S
Frontiers in cellular and infection microbiology 2021;11:756854
Frontiers in cellular and infection microbiology 2021;11:756854
Human newborn bacille Calmette-Guérin vaccination and risk of tuberculosis disease: a case-control study.
Fletcher HA, Filali-Mouhim A, Nemes E, Hawkridge A, Keyser A, Njikan S, Hatherill M, Scriba TJ, Abel B, Kagina BM, Veldsman A, Agudelo NM, Kaplan G, Hussey GD, Sekaly RP, Hanekom WA, BCG study team
BMC medicine 2016 May 16;14:76
BMC medicine 2016 May 16;14:76
Human decidual tissue contains differentiated CD8+ effector-memory T cells with unique properties.
Tilburgs T, Schonkeren D, Eikmans M, Nagtzaam NM, Datema G, Swings GM, Prins F, van Lith JM, van der Mast BJ, Roelen DL, Scherjon SA, Claas FH
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 1;185(7):4470-7
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 1;185(7):4470-7
Only a subset of phosphoantigen-responsive gamma9delta2 T cells mediate protective tuberculosis immunity.
Spencer CT, Abate G, Blazevic A, Hoft DF
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4471-84
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 1;181(7):4471-84
Human NKT cells express granulysin and exhibit antimycobacterial activity.
Gansert JL, Kiessler V, Engele M, Wittke F, Röllinghoff M, Krensky AM, Porcelli SA, Modlin RL, Stenger S
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 15;170(6):3154-61
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 15;170(6):3154-61
T-cell release of granulysin contributes to host defense in leprosy.
Ochoa MT, Stenger S, Sieling PA, Thoma-Uszynski S, Sabet S, Cho S, Krensky AM, Rollinghoff M, Nunes Sarno E, Burdick AE, Rea TH, Modlin RL
Nature medicine 2001 Feb;7(2):174-9
Nature medicine 2001 Feb;7(2):174-9
Biosynthesis of granulysin, a novel cytolytic molecule.
Hanson DA, Kaspar AA, Poulain FR, Krensky AM
Molecular immunology 1999 May;36(7):413-22
Molecular immunology 1999 May;36(7):413-22
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC then fixed and permeabilized and stained with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human Granulysin PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with CD56 Monoclonal Antibody, APC (Product # 17-0567-42) and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or Granulysin Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Decreased frequencies of gammadelta T cells expressing cytotoxic markers in LTB comorbidities. PBMCs were either untreated or treated with Mtb or positive control antigens for 18 h. The absolute (unstimulated, UNS) and antigen-stimulated (PPD, WCL, P/I) net frequencies of cytotoxic (PFN, GZEB, GNLYSN) markers were shown in LTB DM (n = 20), LTB PDM (n = 20), and LTB NDM (n = 20) groups. Geometric mean values were represented using bars, and every circle denotes a single individual. Kruskal-Wallis test with multiple Dunn's comparison were used to determine the p values.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Effect BCG-Nanocage on e x vivo activation and expansion of Vgamma2+ T cells in PBMC from non-human primates. A Typical flow cytometry panel for Vgamma2+ cells gated in T cells from PBMC with or without (co-)stimulation using BCG/IL2, BCG-Nanocage/IL2 or IL2 for 6 days. B Effects of (co-)stimulation using BCG/IL2, BCG-Nanocage/IL2 or IL2 on Vgamma2+ T cells after 6 day treatment, n = 3, *p < 0.05. C Typical flow cytometry panel for Granzyme B+ Perforin+ cells gated in Vgamma2+ T cells in total T cells from PBMC with or without (co-)stimulation using BCG/IL2, BCG-Nanocage/IL2 or IL2 for 6 days. D Effects of (co-)stimulation using BCG/IL2, BCG-Nanocage/IL2 or IL2 on Vgamma2+ Granzyme B+ Perforin+ cells in T cells after 6 day treatment, n = 3, *p < 0.05. E Typical flow cytometry panel for Granulysin + IFN-gamma + cells gated in Vgamma2+ T cells from PBMC with or without (co-)stimulation using BCG/IL2, BCG-Nanocage/IL2 or IL2 for 6 days. F Effects of (co-)stimulation using BCG/IL2, BCG-Nanocage/IL2 or IL2 on Vgamma2+ Granulysin + IFN-gamma+ cells in T cells after 6 day treatment, n = 3, *p < 0.05
- Conjugate
- Yellow dye
