PA1-30417

antibody from Invitrogen Antibodies

Targeting: CASP2 ICH1, MGC2181, NEDD2, PPP1R57

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

CASP2 protein structure - PA1-30417 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Caspase 2 was achieved by transfecting SH-SY5Y with Caspase 2 specific siRNA (Silencer® select Product # S2410). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Caspase 2 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Caspase 2 Polyclonal Antibody (Product # PA1-30417, 1:500 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Caspase 2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Caspase 2 Polyclonal Antibody(Product # PA1-30417) and a 50kDa band corresponding to Caspase 2 was observed across the cell lines tested. Whole Cell Extract (1% SDS) (30 µg lysate) of Jurkat (Lane 1), A-431 (Lane 2), MOLT-4 (Lane 3), SW480 (Lane 4) and SH-SY5Y (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).