697002

antibody from BioLegend

Targeting: CASP2 ICH1, MGC2181, NEDD2, PPP1R57

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

697002

Provider

BioLegend

Proper citation

BioLegend Cat#697002, RRID:AB_2687108

Product name

Purified anti-Caspase-2L

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16017A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from EL-4, NTERA-2 and Jurkat were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-Caspase-2L (clone W16017A) antibody (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti-Caspase-2L antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH antibody. Lane M: molecular weight ladder.

Conjugate

Unconjugated