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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34819 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntenin 1 Recombinant Rabbit Monoclonal Antibody (JE40-72)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A549, SH-SY-5Y, SiHa, human tonsil tissue, human colon tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE40-72
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Syntenin in A549 cell. Samples were incubated with Syntenin monoclonal antibody (Product # MA5-34819), at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Syntenin in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Syntenin monoclonal antibody (Product # MA5-34819), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Syntenin in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Syntenin monoclonal antibody (Product # MA5-34819), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Syntenin in paraffin-embedded human tonsil tissue. Samples were incubated with Syntenin monoclonal antibody (Product # MA5-34819), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Syntenin in paraffin-embedded human colon tissue. Samples were incubated with Syntenin monoclonal antibody (Product # MA5-34819), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Syntenin in A549 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Syntenin monoclonal antibody (Product # MA5-34819) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
