Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-18595 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntenin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, mouse and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles.
Bobis-Wozowicz S, Kmiotek K, Kania K, Karnas E, Labedz-Maslowska A, Sekula M, Kedracka-Krok S, Kolcz J, Boruczkowski D, Madeja Z, Zuba-Surma EK
Journal of molecular medicine (Berlin, Germany) 2017 Feb;95(2):205-220
Journal of molecular medicine (Berlin, Germany) 2017 Feb;95(2):205-220
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of HEK293 cell lysate using Product # PA5-18595 at a concentration of 0.01 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Characteristic of EVs derived from UC-MSCs cultured in xeno-free media (UC-MSC-EVs). a Size analysis of EVs using qNano system (Izon Science Ltd). Representative image is shown. b Western blot analysis of selected proteins in UC-MSC-EVs. Three hundred micrograms of protein extracts was used to detect expression of transmembrane (CD63) and cytosolic (syntenin) proteins. Expression of beta-actin was used as control. c Transcript level for extracellular protein (IL-8) measured by RT-qPCR in UC-MSC-EVs. d Surface antigen profile of UC-MSC-EVs by high-sensitivity flow cytometry. The EV samples were stained with the SYTO(r) RNASelect(tm) Green Fluorescent Cell Stain (Molecular Probes) and selected antibodies labeled with a fluorochrome and further analyzed on an A50-Micro Flow Cytometer (Apogee Flow Systems). The percentage of particles positive for indicated surface marker was analyzed from SYTO(r) RNASelect(tm)-positive objects (in gate R1). Representative dot plots for M1-EVs are shown. e Analysis of transcript levels for genes involved in the maintenance of pluripotency ( NANOG ) or differentiation toward cardiac ( GATA4 ) and endothelial lineage ( FLK1 ) performed with the real time PCR method in UC-MSC-EVs. f Relative transcript levels in EVs compared to parental UC-MSCs. Results are shown as mean +- SD. Results were compared with one-way ANOVA and Dunnet's post hoc test, relative to control conditions ( M6 ). * p < 0.05. UC-MSC umbilical cord-derived mesenchymal