Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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Validation data
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- Product number
- 600-401-P10 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-P10, RRID:AB_2614468
- Product name
- Anti-Histone H3 [Monomethyl Lys79] (RABBIT) Antibody - 600-401-P10
- Antibody type
- Polyclonal
- Vial size
- 50 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Dot Blot of Rabbit Histone H3 [monomethyl Lys79] Antibody. Lane 1: K79 unmodified. Lane 2: K79-Me1. Lane 3: K79-Me2. Lane 4: K79-Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 K79-Me1 antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
- Validation comment
- Dot Blot