Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003817-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003817-M03, RRID:AB_1111925
- Product name
- KLK2 monoclonal antibody (M03), clone 3C5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant KLK2.
- Antigen sequence
MWDLVLSIALSVGCTGAVPLIQSRIVGGWECEKHS
QPWQVAVYSHGWAHCGGVLVHPQWVLTAAHCLKKN
SQVWLGRHNLFEPEDTGQRVPVSHSFPHPLYNMSL
LKHQSLRPDEDSSHDLMLLRLSEPAKITDVVKVLG
LPTQEPALGTTCYASGWGSIEPEEFLRPRSLQCVS
LHLLSNDMCARAYSEKVTEFMLCAGLWTGGKDTCG
GDSGGPLVCNGVLQGITSWGPEPCALPEKPAVYTK
VVHYRKWIKDTIAANP- Isotype
- IgG
- Antibody clone number
- 3C5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KLK2 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to KLK2 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of KLK2 transfected lysate using anti-KLK2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with KLK2 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol