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Learn10195-1-AP
antibody from Proteintech Group
Targeting: RUVBL2
ECP51, INO80J, Reptin52, RVB2, TIH2, TIP48, TIP49b
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 10195-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10195-1-AP, RRID:AB_2184679
- Product name
- RUVBL2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RUVBL2 antibody (Cat. #10195-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis.
NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells.
Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.
Circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.
HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.
Identification and characterization of R2TP in the development of oral squamous cell carcinoma.
WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.
Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.
Multilevel regulation of RUVBL2 expression predicts poor prognosis in hepatocellular carcinoma.
LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.
ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.
RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.
PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading.
Decoding the full picture of Raf1 function based on its interacting proteins.
SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.
Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.
Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.
LINKIN, a new transmembrane protein necessary for cell adhesion.
Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.
Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.
The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery.
Fu Y, Li L, Zhang X, Deng Z, Wu Y, Chen W, Liu Y, He S, Wang J, Xie Y, Tu Z, Lyu Y, Wei Y, Wang S, Cui CP, Liu CH, Zhang L
Nature communications 2024 Apr 6;15(1):2974
Nature communications 2024 Apr 6;15(1):2974
NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells.
Aliyaskarova U, Baiken Y, Renaud F, Couve S, Kisselev AF, Saparbaev M, Groisman R
Scientific reports 2023 Mar 30;13(1):5174
Scientific reports 2023 Mar 30;13(1):5174
Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.
Zhang J, Gan Y, Li H, Yin J, He X, Lin L, Xu S, Fang Z, Kim BW, Gao L, Ding L, Zhang E, Ma X, Li J, Li L, Xu Y, Horne D, Xu R, Yu H, Gu Y, Huang W
Nature communications 2022 May 20;13(1):2835
Nature communications 2022 May 20;13(1):2835
Circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.
Chen Z, Lu T, Huang L, Wang Z, Yan Z, Guan Y, Hu W, Fan Z, Zhu P
The Journal of clinical investigation 2021 Oct 1;131(19)
The Journal of clinical investigation 2021 Oct 1;131(19)
HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.
Nakamura A, Kakihara Y, Funayama A, Haga K, Mikami T, Kobayashi D, Yoshida Y, Izumi K, Kobayashi T, Saeki M
Biochemical and biophysical research communications 2021 Jun 11;557:294-301
Biochemical and biophysical research communications 2021 Jun 11;557:294-301
Identification and characterization of R2TP in the development of oral squamous cell carcinoma.
Kiguchi T, Kakihara Y, Yamazaki M, Katsura K, Izumi K, Tanuma JI, Saku T, Takagi R, Saeki M
Biochemical and biophysical research communications 2021 Apr 9;548:161-166
Biochemical and biophysical research communications 2021 Apr 9;548:161-166
WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.
Dai J, Jiang L, Qiu L, Shao Y, Shi P, Li J
OncoTargets and therapy 2020;13:7033-7044
OncoTargets and therapy 2020;13:7033-7044
Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.
Shin SH, Lee JS, Zhang JM, Choi S, Boskovic ZV, Zhao R, Song M, Wang R, Tian J, Lee MH, Kim JH, Jeong M, Lee JH, Petukhov M, Lee SW, Kim SG, Zou L, Byun S
Science advances 2020 Jul;6(31):eaay9131
Science advances 2020 Jul;6(31):eaay9131
Multilevel regulation of RUVBL2 expression predicts poor prognosis in hepatocellular carcinoma.
Yan T, Liu F, Gao J, Lu H, Cai J, Zhao X, Sun Y
Cancer cell international 2019;19:249
Cancer cell international 2019;19:249
LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.
Wang Y, Zhu P, Luo J, Wang J, Liu Z, Wu W, Du Y, Ye B, Wang D, He L, Ren W, Wang J, Sun X, Chen R, Tian Y, Fan Z
The EMBO journal 2019 Sep 2;38(17):e101110
The EMBO journal 2019 Sep 2;38(17):e101110
ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer.
Harwood FC, Klein Geltink RI, O'Hara BP, Cardone M, Janke L, Finkelstein D, Entin I, Paul L, Houghton PJ, Grosveld GC
Science advances 2018 Sep;4(9):eaar3938
Science advances 2018 Sep;4(9):eaar3938
RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.
Trotman JB, Agana BA, Giltmier AJ, Wysocki VH, Schoenberg DR
The Journal of biological chemistry 2018 Oct 26;293(43):16596-16607
The Journal of biological chemistry 2018 Oct 26;293(43):16596-16607
PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading.
Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, Busino L, Rothenberg E, Pagano M
eLife 2018 Jul 9;7
eLife 2018 Jul 9;7
Decoding the full picture of Raf1 function based on its interacting proteins.
Huang Y, Guo XX, Han B, Zhang XM, An S, Zhang XY, Yang Y, Liu Y, Hao Q, Xu TR
Oncotarget 2017 Sep 15;8(40):68329-68337
Oncotarget 2017 Sep 15;8(40):68329-68337
SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.
Gribling-Burrer AS, Leichter M, Wurth L, Huttin A, Schlotter F, Troffer-Charlier N, Cura V, Barkats M, Cavarelli J, Massenet S, Allmang C
Nucleic acids research 2017 May 19;45(9):5399-5413
Nucleic acids research 2017 May 19;45(9):5399-5413
Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.
Malinová A, Cvačková Z, Matějů D, Hořejší Z, Abéza C, Vandermoere F, Bertrand E, Staněk D, Verheggen C
The Journal of cell biology 2017 Jun 5;216(6):1579-1596
The Journal of cell biology 2017 Jun 5;216(6):1579-1596
Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.
Bizarro J, Charron C, Boulon S, Westman B, Pradet-Balade B, Vandermoere F, Chagot ME, Hallais M, Ahmad Y, Leonhardt H, Lamond A, Manival X, Branlant C, Charpentier B, Verheggen C, Bertrand E
The Journal of cell biology 2014 Nov 24;207(4):463-80
The Journal of cell biology 2014 Nov 24;207(4):463-80
LINKIN, a new transmembrane protein necessary for cell adhesion.
Kato M, Chou TF, Yu CZ, DeModena J, Sternberg PW
eLife 2014 Dec 1;3:e04449
eLife 2014 Dec 1;3:e04449
Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.
Kim SG, Hoffman GR, Poulogiannis G, Buel GR, Jang YJ, Lee KW, Kim BY, Erikson RL, Cantley LC, Choo AY, Blenis J
Molecular cell 2013 Jan 10;49(1):172-85
Molecular cell 2013 Jan 10;49(1):172-85
Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.
Machado-Pinilla R, Liger D, Leulliot N, Meier UT
RNA (New York, N.Y.) 2012 Oct;18(10):1833-45
RNA (New York, N.Y.) 2012 Oct;18(10):1833-45
The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery.
Boulon S, Marmier-Gourrier N, Pradet-Balade B, Wurth L, Verheggen C, Jády BE, Rothé B, Pescia C, Robert MC, Kiss T, Bardoni B, Krol A, Branlant C, Allmang C, Bertrand E, Charpentier B
The Journal of cell biology 2008 Feb 11;180(3):579-95
The Journal of cell biology 2008 Feb 11;180(3):579-95
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10195-1-AP(RUVBL2 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP result of anti-RUVBL2(10195-1-AP for IP and Detection).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 10195-1-AP(RUVBL2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human testis using 10195-1-AP(RUVBL2 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
