TA504384

antibody from OriGene

Targeting: RUVBL2 ECP51, INO80J, Reptin52, RVB2, TIH2, TIP48, TIP49b

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504384

Provider

OriGene

Proper citation

OriGene Cat#TA504384, RRID:AB_2622433

Product name

RUVBL2 (Reptin/TIP49B) mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)

Antibody type

Monoclonal

Description

RUVBL2 (Reptin/TIP49B) mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

RUVBL2

Isotype

IgG

Antibody clone number

OTI2B9

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RUVBL2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (RC200933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200933 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RUVBL2 antibody(TA504384), and then analyzed by flow cytometry.

Validation comment

FC