BAM4236
antibody from R&D Systems
Targeting: P4HB
DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PO4DB, PO4HB, PROHB
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BAM4236 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Protein Disulfide Isomerase/P4HB Biotinylated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Conjugated Protein Disulfide Isomerase/P4HB antibodies are ideal for immunocytochemistry colocalization studies in the lumen of the endoplasmic reticulum. The unconjugated antibody detects human Protein Disulfide Isomerase/P4HB in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Antigen sequence
P07237
- Isotype
- IgG
- Antibody clone number
- 537331
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Protein Disulfide Isomerase/P4HB in HeLa Human Cell Line. Protein Disulfide Isomerase/P4HB was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Protein Disulfide Isomerase/P4HB Biotinylated Monoclonal Antibody (Catalog # BAM4236) at 25 µg/mL overnight at 4 ° C. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.