ABIN1882265

antibody from antibodies-online

Targeting: MGMT

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1882265

Provider

antibodies-online

Product name

anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibody

Antibody type

Monoclonal

Antigen

Purified His-tagged MGMT protein was used to produced this monoclonal antibody.

Description

Ascites

Reactivity

Human

Host

Mouse

Epitope

AA 1-207

Isotype

IgG

Antibody clone number

888CT22-2-4

Vial size

100 μL

References

Submitted references

The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity.

Edara S, Goodtzova K, Pegg AE
Carcinogenesis 1995 Jul;16(7):1637-42

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Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase.

Koike G, Maki H, Takeya H, Hayakawa H, Sekiguchi M
The Journal of biological chemistry 1990 Sep 5;265(25):14754-62

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cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells.

Rydberg B, Spurr N, Karran P
The Journal of biological chemistry 1990 Jun 5;265(16):9563-9

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Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA.

Hayakawa H, Koike G, Sekiguchi M
Journal of molecular biology 1990 Jun 20;213(4):739-47

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Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine.

Tano K, Shiota S, Collier J, Foote RS, Mitra S
Proceedings of the National Academy of Sciences of the United States of America 1990 Jan;87(2):686-90

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

MGMT Antibody (ABIN1882265) western blot analysis in MCF-7,Jurkat,Hela cell line lysates (35 μg/lane). This demonstrates the MGMT antibody detected the MGMT protein (arrow).