H00004255-D01

antibody from Abnova Corporation

Targeting: MGMT

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004255-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004255-D01, RRID:AB_10721128

Product name

MGMT MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MGMT protein.

Antigen sequence

MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLG
KGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYF
HQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
VKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVP
ILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHR
LGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MGMT MaxPab rabbit polyclonal antibody. Western Blot analysis of MGMT expression in mouse liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MGMT expression in transfected 293T cell line (H00004255-T01) by MGMT MaxPab polyclonal antibody.Lane 1: MGMT transfected lysate(21.6 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of MGMT transfected lysate using anti-MGMT MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MGMT purified MaxPab mouse polyclonal antibody (B01P) (H00004255-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol