Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN953415 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (N-Term), (AA 1-30) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide (between 1-30 aa) - KLH conjugated - corresponding to the N-terminal region of human MGMT.
- Description
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 1-30
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Methylation tolerance due to an O6-methylguanine DNA methyltransferase (MGMT) field defect in the colonic mucosa: an initiating step in the development of mismatch repair-deficient colorectal cancers.
Polymorphic DNA repair and metabolic genes: a multigenic study on gastric cancer.
Comprehensive analysis of DNA repair gene polymorphisms and survival in patients with early stage non-small-cell lung cancer.
Svrcek M, Buhard O, Colas C, Coulet F, Dumont S, Massaoudi I, Lamri A, Hamelin R, Cosnes J, Oliveira C, Seruca R, Gaub MP, Legrain M, Collura A, Lascols O, Tiret E, Fléjou JF, Duval A
Gut 2010 Nov;59(11):1516-26
Gut 2010 Nov;59(11):1516-26
Polymorphic DNA repair and metabolic genes: a multigenic study on gastric cancer.
Palli D, Polidoro S, D'Errico M, Saieva C, Guarrera S, Calcagnile AS, Sera F, Allione A, Gemma S, Zanna I, Filomena A, Testai E, Caini S, Moretti R, Gomez-Miguel MJ, Nesi G, Luzzi I, Ottini L, Masala G, Matullo G, Dogliotti E
Mutagenesis 2010 Nov;25(6):569-75
Mutagenesis 2010 Nov;25(6):569-75
Comprehensive analysis of DNA repair gene polymorphisms and survival in patients with early stage non-small-cell lung cancer.
Kim M, Kang HG, Lee SY, Lee HC, Lee EB, Choi YY, Lee WK, Cho S, Jin G, Jheon HS, Son JW, Lee MH, Jung DK, Cha SI, Kim CH, Kang YM, Kam S, Jung TH, Jheon S, Park JY
Cancer science 2010 Nov;101(11):2436-42
Cancer science 2010 Nov;101(11):2436-42
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of MGMT (arrow) using rabbit polyclonal MGMT antibody Cat.-No. AP52689PU-N (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MGMT gene.