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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- NBP1-32535 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32535, RRID:AB_2171757
- Product name
- Rabbit Polyclonal PKC gamma Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKC gamma Antibody [NBP1-32535] - Sample(30 ug whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:2000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKC gamma Antibody [NBP1-32535] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded Hun7 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded mouse brain. PKC gamma antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded rat brain. PKC gamma antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: PKC gamma Antibody [NBP1-32535] - Frozen-sectioned adult mouse cerebellum. Green: PKC gamma protein stained by PKC gamma antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.
