PA5-27732

PRKCG antibody from Invitrogen Antibodies

MGC57564, PKC?, PKCC, PKCG, SCA14

Western blot

Supportive data in Antibodypedia

Provider product page for PA5-27732

Antibody Data

Product number

PA5-27732

Provider

Invitrogen Antibodies

Product name

Anti-PKC gamma Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

PA5-27732 targets PKC gamma in WB applications and shows reactivity with Human samples. The PA5-27732 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 166 of Human PKC gamma. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.55 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PKC gamma using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC gamma polyclonal antibody (Product # PA5-27732) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PKC gamma was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC gamma Polyclonal Antibody (Product # PA5-27732) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.