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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-27732 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKC gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, mouse brain, rat brain.
- Concentration
- 0.55 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PKC gamma was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC gamma Polyclonal Antibody (Product # PA5-27732) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PKC gamma Polyclonal Antibody (Product # PA5-27732). Sample (30 µg whole cell lysate). A: MOLT4. 7.5% SDS PAGE. PKC gamma Polyclonal Antibody (Product # PA5-27732) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of PKC-gamma was performed in frozen-sectioned adult mouse cerebellum tissue using PKC gamma Polyclonal Antibody (Product # PA5-27732) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
