PA5-28618

antibody from Invitrogen Antibodies

Targeting: PRKCG MGC57564, PKCC, PKCG, PKCγ, SCA14

Antibody data

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Validation data

Product number: PA5-28618 - Provider product page
Provider: Invitrogen Antibodies
Product name: PKC gamma Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, mouse brain, rat brain. Predicted reactivity: Mouse (98%), Rat (98%), Rabbit (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PRKCG protein structure - PA5-28618 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKC gamma using 50 µg mouse brain extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC gamma polyclonal antibody (Product # PA5-28618) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKC gamma using 50 µg rat brain extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC gamma polyclonal antibody (Product # PA5-28618) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKC gamma using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC gamma polyclonal antibody (Product # PA5-28618) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PKC gamma was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC gamma Polyclonal Antibody (Product # PA5-28618) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PKC gamma Polyclonal Antibody (Product # PA5-28618). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma Polyclonal Antibody (Product # PA5-28618) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.