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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [4]
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- Product number
- PA5-28618 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKC gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse brain, rat brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKC gamma using 50 µg mouse brain extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC gamma polyclonal antibody (Product # PA5-28618) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKC gamma using 50 µg rat brain extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC gamma polyclonal antibody (Product # PA5-28618) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKC gamma using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC gamma polyclonal antibody (Product # PA5-28618) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PKC gamma was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC gamma Polyclonal Antibody (Product # PA5-28618) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PKC gamma Polyclonal Antibody (Product # PA5-28618). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma Polyclonal Antibody (Product # PA5-28618) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PKC gamma was performed in paraffin-embedded mouse brain tissue using PKC gamma Polyclonal Antibody (Product # PA5-28618) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PKC gamma was performed in paraffin-embedded rat brain tissue using PKC gamma Polyclonal Antibody (Product # PA5-28618) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of PKC-gamma was performed in frozen-sectioned adult mouse cerebellum tissue using PKC gamma Polyclonal Antibody (Product # PA5-28618) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hun7 xenograft, using PKC gamma (Product # PA5-28618) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
