Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB600-506G - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal alpha Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Drosophila, Simian, Yeast
- Host
- Rat
- Conjugate
- Green dye
- Antigen sequence
A linear sequence requiring an arom
atic residue at the C terminus, wit
h the two adjacent amino acids bein
g negatively charged (represented b
y Gly-Gly-Tyr in Tyr-Tubulin).- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (YL1/2) [DyLight 488] [NB600-506G] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.