Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-1639AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal alpha Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This alpha Tubulin Antibody (YOL1/34) detects an epitope on alpha tubulin between the amino acids 414-422. It has a higher affinity for fixed microtubules (formaldehyde or glutaraldehyde) than native microtubules when performing IHC.
- Reactivity
- Human, Mouse, Rat, Drosophila, Yeast
- Host
- Rat
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.25 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: alpha Tubulin Antibody (YOL1/34) [Alexa Fluor® 647] [NB100-1639AF647] - An intracellular stain was performed on HeLa cells with alpha Tubulin Antibody [YOL1/34] NB100-1639AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.