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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C355203 - Provider product page
- Provider
- LSBio
- Product name
- TUBA1A / Tubulin Alpha 1a Antibody (clone TU-08) LS-C355203
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Porcine
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- TU-08
- Storage
- Store at 2°C to 8°C. Do not freeze.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), PC-3 (Lane 5), PC-12 (Lane 6) and RSC96 (Lane 7). The blot was probed with Anti-alpha/beta Tubulin Mouse Monoclonal Antibody and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A 52 kDa band corresponding to alpha/beta Tubulin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using 12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk.
