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LearnNB600-567
antibody from Novus Biologicals
Targeting: TUBA1A
B-ALPHA-1, FLJ25113, TUBA3
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Immunoelectron microscopy RadioimmunoassayAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB600-567 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-567, RRID:AB_2210202
- Product name
- Mouse Monoclonal alpha Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. This alpha Tubulin [ac Lys40] Antibody (6-11B-1) recognizes an epitope located on the alpha 3 isoform of Chlamydomonas axonemal alpha-tubulin, within four residues of Lys-40 when this amino acid is acetylated
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Porcine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mass Spectrometric Quantitation of Tubulin Acetylation from Pepsin-Digested Rat Brain Tissue Using a Novel Stable-Isotope Standard and Capture by Anti-Peptide Antibody (SISCAPA) Method.
Elevated LIM kinase 1 in nonmetastatic prostate cancer reflects its role in facilitating androgen receptor nuclear translocation.
Yang X, Naughton SX, Han Z, He M, Zheng YG, Terry AV Jr, Bartlett MG
Analytical chemistry 2018 Feb 6;90(3):2155-2163
Analytical chemistry 2018 Feb 6;90(3):2155-2163
Elevated LIM kinase 1 in nonmetastatic prostate cancer reflects its role in facilitating androgen receptor nuclear translocation.
Mardilovich K, Gabrielsen M, McGarry L, Orange C, Patel R, Shanks E, Edwards J, Olson MF
Molecular cancer therapeutics 2015 Jan;14(1):246-58
Molecular cancer therapeutics 2015 Jan;14(1):246-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - Western Blot validation of monoclonal anti-Acetylated Tubulin antibody clone: 6-11B-1 at 1:2000 dilution with goat anti-Mouse IgG-Peroxidase secondary antibodychemiluminescent substrate development. The lysates employed were from cell lines: 1. HeLa, 2. U87, 3. COS7, 4. P19, 5. RAT2, 6. CHO, 7. MDBK and 8. MDCK Alpha tubulin molecular weight: 50 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - Identification of a surrogate peptide YCLEHGIQPDGQMPSDK40(ac)TIGGGDDSF with mass spectrometry. This peptide was immunoprecipitated with 6-11B-1 mAb from peptic digested rat brain tissue lysate. Image submitted via customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: alpha Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - Nerve pathways in the embryonic zebrafish brain and spinal cord revealed by alpha Tubulin (ac Lys40) Antibody (6-11B-1). Immunoperoxidase staining of a whole-mount embyro at 24 hours of development.
