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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB93441 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal alpha Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha Tubulin Antibody (961258) [MAB93441] - Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa, Jurkat, NIH-3T3, Neuro2A and Rat-2 cell lines. PVDF membrane was probed with Mouse alpha Tubulin Antibody (961258) (Catalog # MAB93441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at an approximate molecular weight of 50 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: alpha Tubulin Antibody (961258) [MAB93441] - alpha Tubulin was detected in immersion fixed paraffin-embedded sections of human brin hippocampus tissue using alpha Tubulin Antibody (961258) at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.
