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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-85418 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TUBA1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (98%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Positive Control: 293T, A431, Jurkat, Raji, NIH-3T3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.24 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUBA1A in A) NIH-3T3 cells using TUBA1A polyclonal antibody (Product # PA5-85418) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TUBA1A in A) 293T, B) A431, C) Jurkat, D) Raji cells using TUBA1A polyclonal antibody (Product # PA5-85418) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TUBA1A in 4% paraformaldehyde-fixed HeLa cells using TUBA1A polyclonal antibody (Product # PA5-85418) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TUBA1A in paraffin-embedded mouse testis using TUBA1A polyclonal antibody (Product # PA5-85418) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TUBA1A in paraffin-embedded mouse ovary using TUBA1A polyclonal antibody (Product # PA5-85418) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TUBA1A in paraffin-embedded human breast cancer using TUBA1A polyclonal antibody (Product # PA5-85418) at a dilution of 1:250.
