Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX48492 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48492, RRID:AB_11174492
- Product name
- Plasminogen antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1ml
- Storage
- Store vial at 4° C for short-term use. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Goat anti Plasminogen antibody(GTX48494) was used to detect Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (on 4 C in blocking buffer). Detection shown was using Dylight 649 conjugated Donkey anti goat (1:10K in TBS/blocking buffer) 1 hr RT.
- Validation comment
- WB