Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C149920 - Provider product page
- Provider
- LSBio
- Product name
- Anti-CCNE1 / Cyclin E1 Antibody LS-C149920
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 25µg
- Storage
- Store at -20 to -70 C. Stable for 12 months from date of receipt.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Cyclin E1 in Human Breast. Cyclin E1 was detected in immersion fixed paraffin-embedded sections of human breast using Sheep Anti-Human Cyclin E1 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.