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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Flow cytometry [1]
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- Product number
- MAB12242 - Provider product page
- Provider
- Abnova Corporation
- Product name
- CCNE1 monoclonal antibody, clone 5F8C5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant human CCNE1.
- Isotype
- IgG
- Antibody clone number
- 5F8C5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human CCNE1 (AA:307-410) recombinant protein (Expected MW is 37.5 kDa) with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Hela cell lysate; Lane 2: K562 cell lysate; Lane 3: NIH/3T3 cell lysate; Lane 4: C6 cell lysate; Lane 5: MCF-7 cell lysate; Lane 6: Jurkat cell lysate; Lane 7: A431 cell lysate with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CCNE1 (AA: 307-410)-hIgGFc transfected HEK293 cells] with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of K562 cells with CCNE1 monoclonal antibody, clone 5F8C5 (Cat# MAB12242) at 1:200-1:400 dilution (Green) and negative control (Red).
- Validation comment
- Flow Cytometry
