Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- 13-9714-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin E Monoclonal Antibody (HE12), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody HE12 recognizes human Cyclin E. Cyclins regulate the cell cycle by binding and activating cyclin-dependent kinases (CDK). Cyclin E regulates the transition from G1 to S phase by binding and activating CDK2. The Cyclin E/CDK2 complex is responsible for the phosphorylation of downstream proteins that facilitate the G1 to S phase transition. Overexpression of Cyclin E is found in several types of cancer including breast, colon, bladder, lung and skin. In breast cancer, Cyclin E overexpression is associated with increased stage and grade as well as poor prognosis. Applications Reported: This HE12 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This HE12 antibody has been tested by immunohistochemistry of FFPE human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- HE12
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human testes tissue using 20 µg/mL of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (left) or 20 µg/mL of Anti-Human Cyclin E Biotin (right) followed by Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
- Conjugate
- Biotin