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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-16237 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Smoothened controls cyclin D2 expression and regulates the generation of intermediate progenitors in the developing cortex.
Komada M, Iguchi T, Takeda T, Ishibashi M, Sato M
Neuroscience letters 2013 Jun 28;547:87-91
Neuroscience letters 2013 Jun 28;547:87-91
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Cyclin E using Cyclin E Polyclonal Antibody (Product # PA5-16237) on SKBR3 Cells and MAD109 Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cyclin E showing staining in the nucleus of paraffin-embedded human astroglioma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclin E Rabbit Polyclonal Antibody (Product # PA5-16237) diluted in 3% BSA-PBS at a dilution of 1:50 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cyclin E showing staining in the nucleus and weak cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclin E Rabbit Polyclonal Antibody (Product # PA5-16237) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Cyclin E using Cyclin E Polyclonal Antibody (Product # PA5-16237) on denatured Human SKBR3 Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Cyclin E using Cyclin E Polyclonal Antibody (Product # PA5-16237) on Native Human LS174T Cells.
