MA5-14333

antibody from Invitrogen Antibodies

Targeting: CCNE1 CCNE

Western blot

Immunocytochemistry

Antibody data

Submit

Validation data

Product number: MA5-14333 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-Cyclin E Monoclonal Antibody (HE12), Biotin
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-14336 targets Cyclin E in FACS, IF, IHC (P), and WB applications and shows reactivity with Human and Mouse samples. The MA5-14336 immunogen is recombinant human cyclin E protein.
Reactivity: Human, Mouse
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: HE12
Vial size: 500 µL
Concentration: 0.2 mg/ml
Storage: 4° C

CCNE1 protein structure - MA5-14333 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Cyclin E in C2C12 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Cyclin E monoclonal antibody (Product # MA5-14336) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Cyclin E in Jurkat cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Cyclin E monoclonal antibody (Product # MA5-14336) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Cyclin E in MCF-7 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Cyclin E monoclonal antibody (Product # MA5-14336) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin