HPA021241

antibody from Atlas Antibodies

Targeting: PHGDH PDG, PGDH, SERA

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA021241

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA021241, RRID:AB_1855299

Product name

Anti-PHGDH

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human PHGDH, Gene description: phosphoglycerate dehydrogenase, Alternative Gene Names: PDG, PGDH, SERA, Validated applications: ICC, IHC, WB, Uniprot ID: O43175, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells

Walvekar A, Warmoes M, Cheung D, Sikora T, Seyedkatouli N, Gomez-Giro G, Perrone S, Dengler L, Unger F, Santos B, Gavotto F, Dong X, Becker-Kettern J, Kwon Y, Jäger C, Schwamborn J, Van Bergen N, Christodoulou J, Linster C
Cellular & Molecular Biology Letters 2025;30(1)

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Central carbon metabolism exhibits unique characteristics during the handling of fungal patterns by monocyte-derived dendritic cells

Alvarez Y, Mancebo C, Alonso S, Montero O, Fernández N, Sánchez Crespo M
Redox Biology 2024;73

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ASCT2 is a major contributor to serine uptake in cancer cells

Conger K, Chidley C, Ozgurses M, Zhao H, Kim Y, Semina S, Burns P, Rawat V, Lietuvninkas L, Sheldon R, Ben-Sahra I, Frasor J, Sorger P, DeNicola G, Coloff J
Cell Reports 2024;43(8):114552

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High expression of phosphoglycerate dehydrogenase predicts poor outcome in patients with high-grade serous ovarian cancer

van Wagensveld L, Van Nyen T, Annibali D, Sonke G, Kruitwagen R, Amant F, Horlings H
The Oncologist 2024;29(9):e1231-e1234

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Jasani N, Xu X, Posorske B, Kim Y, Vera O, Tsai K, DeNicola G, Karreth F
2024

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Extracellular Vesicle Secretion by Leukemia Cells In Vivo Promotes CLL Progression by Hampering Antitumor T-cell Responses

Gargiulo E, Viry E, Morande P, Largeot A, Gonder S, Xian F, Ioannou N, Benzarti M, Kleine Borgmann F, Mittelbronn M, Dittmar G, Nazarov P, Meiser J, Stamatopoulos B, Ramsay A, Moussay E, Paggetti J
Blood Cancer Discovery 2023;4(1):54-77

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Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate

Wu Y, Zhang C, Xiong J, Cai D, Wang C, Wang Y, Liu Y, Wang Y, Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu H, Guo H, Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, Lin S, Piao H, Zhou J, Lin S
Cell Research 2023;33(11):835-850

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MicroRNA regulation of the serine synthesis pathway in endocrine-resistant breast cancer cells

Petri B, Piell K, Wilt A, Howser A, Winkler L, Whitworth M, Valdes B, Lehman N, Clem B, Klinge C
Endocrine-Related Cancer 2023;30(11)

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p63 orchestrates serine and one carbon metabolism enzymes expression in head and neck cancer

Cappello A, Tosetti G, Smirnov A, Ganini C, Yang X, Shi Y, Wang Y, Melino G, Bernassola F, Candi E
Biology Direct 2023;18(1)

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PHGDH-mediated endothelial metabolism drives glioblastoma resistance to chimeric antigen receptor T cell immunotherapy.

Zhang D, Li AM, Hu G, Huang M, Yang F, Zhang L, Wellen KE, Xu X, Conn CS, Zou W, Kahn M, Rhoades SD, Weljie AM, Fuchs SY, Amankulor N, Yoshor D, Ye J, Koumenis C, Gong Y, Fan Y
Cell metabolism 2023 Mar 7;35(3):517-534.e8

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An LKB1–mitochondria axis controls TH17 effector function

Baixauli F, Piletic K, Puleston D, Villa M, Field C, Flachsmann L, Quintana A, Rana N, Edwards-Hicks J, Matsushita M, Stanczak M, Grzes K, Kabat A, Fabri M, Caputa G, Kelly B, Corrado M, Musa Y, Duda K, Mittler G, O’Sullivan D, Sesaki H, Jenuwein T, Buescher J, Pearce E, Sanin D, Pearce E
Nature 2022;610(7932):555-561

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PHGDH heterogeneity potentiates cancer cell dissemination and metastasis

Rossi M, Altea-Manzano P, Demicco M, Doglioni G, Bornes L, Fukano M, Vandekeere A, Cuadros A, Fernández-García J, Riera-Domingo C, Jauset C, Planque M, Alkan H, Nittner D, Zuo D, Broadfield L, Parik S, Pane A, Rizzollo F, Rinaldi G, Zhang T, Teoh S, Aurora A, Karras P, Vermeire I, Broekaert D, Elsen J, Knott M, Orth M, Demeyer S, Eelen G, Dobrolecki L, Bassez A, Brussel T, Sotlar K, Lewis M, Bartsch H, Wuhrer M, Veelen P, Carmeliet P, Cools J, Morrison S, Marine J, Lambrechts D, Mazzone M, Hannon G, Lunt S, Grünewald T, Park M, Rheenen J, Fendt S
Nature 2022;605(7911):747-753

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Lineage-specific silencing of PSAT1 induces serine auxotrophy and sensitivity to dietary serine starvation in luminal breast tumors

Choi B, Rawat V, Högström J, Burns P, Conger K, Ozgurses M, Patel J, Mehta T, Warren A, Selfors L, Muranen T, Coloff J
Cell Reports 2022;38(3):110278

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Glycolysis Dependency as a Hallmark of SF3B1-Mutated Cells

Vivet-Noguer R, Tarin M, Canbezdi C, Dayot S, Silva L, Houy A, Martineau S, Mieulet V, Gentric G, Loew D, Lombard B, Nemati F, Richon S, Guyonnet L, Servois V, Vagner S, Stern M, Roman-Roman S, Alsafadi S
Cancers 2022;14(9):2113

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Mitochondria preserve an autarkic one-carbon cycle to confer growth-independent cancer cell migration and metastasis

Kiweler N, Delbrouck C, Pozdeev V, Neises L, Soriano-Baguet L, Eiden K, Xian F, Benzarti M, Haase L, Koncina E, Schmoetten M, Jaeger C, Noman M, Vazquez A, Janji B, Dittmar G, Brenner D, Letellier E, Meiser J
Nature Communications 2022;13(1)

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Phosphoglycerate dehydrogenase positively regulates the proliferation of chicken muscle cells

Wang H, Hu M, Ding Z, Zhou X, Yang S, Shen Z, Yan F, Zhao A
Poultry Science 2022;101(5):101805

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Serine metabolism remodeling after platinum-based chemotherapy identifies vulnerabilities in a subgroup of resistant ovarian cancers.

Van Nyen T, Planque M, van Wagensveld L, Duarte JAG, Zaal EA, Talebi A, Rossi M, Körner PR, Rizzotto L, Moens S, De Wispelaere W, Baiden-Amissah REM, Sonke GS, Horlings HM, Eelen G, Berardi E, Swinnen JV, Berkers CR, Carmeliet P, Lambrechts D, Davidson B, Agami R, Fendt SM, Annibali D, Amant F
Nature communications 2022 Aug 5;13(1):4578

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In Vivo Evidence for Serine Biosynthesis-Defined Sensitivity of Lung Metastasis, but Not of Primary Breast Tumors, to mTORC1 Inhibition

Rinaldi G, Pranzini E, Van Elsen J, Broekaert D, Funk C, Planque M, Doglioni G, Altea-Manzano P, Rossi M, Geldhof V, Teoh S, Ross C, Hunter K, Lunt S, Grünewald T, Fendt S
Molecular Cell 2021;81(2):386-397.e7

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Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin

Byles V, Cormerais Y, Kalafut K, Barrera V, Hughes Hallett J, Sui S, Asara J, Adams C, Hoxhaj G, Ben-Sahra I, Manning B
Molecular Metabolism 2021;53

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ATF3 promotes the serine synthesis pathway and tumor growth under dietary serine restriction

Li X, Gracilla D, Cai L, Zhang M, Yu X, Chen X, Zhang J, Long X, Ding H, Yan C
Cell Reports 2021;36(12):109706

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Metabolic compensation activates pro-survival mTORC1 signaling upon 3-phosphoglycerate dehydrogenase inhibition in osteosarcoma

Rathore R, Caldwell K, Schutt C, Brashears C, Prudner B, Ehrhardt W, Leung C, Lin H, Daw N, Beird H, Giles A, Wang W, Lazar A, Chrisinger J, Livingston J, Van Tine B
Cell Reports 2021;34(4):108678

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Exercise-induced angiogenesis is dependent on metabolically primed ATF3/4+ endothelial cells

Fan Z, Turiel G, Ardicoglu R, Ghobrial M, Masschelein E, Kocijan T, Zhang J, Tan G, Fitzgerald G, Gorski T, Alvarado-Diaz A, Gilardoni P, Adams C, Ghesquière B, De Bock K
Cell Metabolism 2021;33(9):1793-1807.e9

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Enzymatic activation of pyruvate kinase increases cytosolic oxaloacetate to inhibit the Warburg effect

Wiese E, Hitosugi S, Loa S, Sreedhar A, Andres-Beck L, Kurmi K, Pang Y, Karnitz L, Gonsalves W, Hitosugi T
Nature Metabolism 2021;3(7):954-968

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Serine biosynthesis defect due to haploinsufficiency of PHGDH causes retinal disease

Eade K, Gantner M, Hostyk J, Nagasaki T, Giles S, Fallon R, Harkins-Perry S, Baldini M, Lim E, Scheppke L, Dorrell M, Cai C, Baugh E, Wolock C, Wallace M, Berlow R, Goldstein D, Metallo C, Friedlander M, Allikmets R
Nature Metabolism 2021;3(3):366-377

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Serine synthesis influences tamoxifen response in ER+ human breast carcinoma

Metcalf S, Petri B, Kruer T, Green B, Dougherty S, Wittliff J, Klinge C, Clem B
Endocrine-Related Cancer 2021;28(1):27-37

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One carbon metabolism in human lung cancer.

Yao S, Peng L, Elakad O, Küffer S, Hinterthaner M, Danner BC, von Hammerstein-Equord A, Ströbel P, Bohnenberger H
Translational lung cancer research 2021 Jun;10(6):2523-2538

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Serine synthesis pathway inhibition cooperates with dietary serine and glycine limitation for cancer therapy.

Tajan M, Hennequart M, Cheung EC, Zani F, Hock AK, Legrave N, Maddocks ODK, Ridgway RA, Athineos D, Suárez-Bonnet A, Ludwig RL, Novellasdemunt L, Angelis N, Li VSW, Vlachogiannis G, Valeri N, Mainolfi N, Suri V, Friedman A, Manfredi M, Blyth K, Sansom OJ, Vousden KH
Nature communications 2021 Jan 14;12(1):366

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PHGDH supports liver ceramide synthesis and sustains lipid homeostasis

Kang Y, Falzone A, Liu M, González-Sánchez P, Choi B, Coloff J, Saller J, Karreth F, DeNicola G
Cancer & Metabolism 2020;8(1)

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Inverse Data-Driven Modeling and Multiomics Analysis Reveals Phgdh as a Metabolic Checkpoint of Macrophage Polarization and Proliferation

Wilson J, Nägele T, Linke M, Demel F, Fritsch S, Mayr H, Cai Z, Katholnig K, Sun X, Fragner L, Miller A, Haschemi A, Popa A, Bergthaler A, Hengstschläger M, Weichhart T, Weckwerth W
Cell Reports 2020;30(5):1542-1552.e7

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Characterization of PHGDH expression in bladder cancer: potential targeting therapy with gemcitabine/cisplatin and the contribution of promoter DNA hypomethylation

Yoshino H, Enokida H, Osako Y, Nohata N, Yonemori M, Sugita S, Kuroshima K, Tsuruda M, Tatarano S, Nakagawa M
Molecular Oncology 2020;14(9):2190-2202

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Structure–Activity Relationships (SARs) of α-Ketothioamides as Inhibitors of Phosphoglycerate Dehydrogenase (PHGDH)

Spillier Q, Ravez S, Unterlass J, Corbet C, Degavre C, Feron O, Frédérick R
Pharmaceuticals 2020;13(2):20

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Macrophages induce malignant traits in mammary epithelium via IKKε/TBK1 kinases and the serine biosynthesis pathway

Wilcz‐Villega E, Carter E, Ironside A, Xu R, Mataloni I, Holdsworth J, Jones W, Moreno Béjar R, Uhlik L, Bentham R, Godinho S, Dalli J, Grose R, Szabadkai G, Jones L, Hodivala‐Dilke K, Bianchi K
EMBO Molecular Medicine 2020;12(2)

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Limited Environmental Serine and Glycine Confer Brain Metastasis Sensitivity to PHGDH Inhibition

Ngo B, Kim E, Osorio-Vasquez V, Doll S, Bustraan S, Liang R, Luengo A, Davidson S, Ali A, Ferraro G, Fischer G, Eskandari R, Kang D, Ni J, Plasger A, Rajasekhar V, Kastenhuber E, Bacha S, Sriram R, Stein B, Bakhoum S, Snuderl M, Cotzia P, Healey J, Mainolfi N, Suri V, Friedman A, Manfredi M, Sabatini D, Jones D, Yu M, Zhao J, Jain R, Keshari K, Davies M, Vander Heiden M, Hernando E, Mann M, Cantley L, Pacold M
Cancer Discovery 2020;10(9):1352-1373

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ILF3 is a substrate of SPOP for regulating serine biosynthesis in colorectal cancer

Li K, Wu J, Qin B, Fan Z, Tang Q, Lu W, Zhang H, Xing F, Meng M, Zou S, Wei W, Chen H, Cai J, Wang H, Zhang H, Cai J, Fang L, Bian X, Chen C, Lan P, Ghesquière B, Fang L, Lee M
Cell Research 2019;30(2):163-178

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Increased Serine Synthesis Provides an Advantage for Tumors Arising in Tissues Where Serine Levels Are Limiting

Sullivan M, Mattaini K, Dennstedt E, Nguyen A, Sivanand S, Reilly M, Meeth K, Muir A, Darnell A, Bosenberg M, Lewis C, Vander Heiden M
Cell Metabolism 2019;29(6):1410-1421.e4

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Increased PHGDH expression promotes aberrant melanin accumulation

Mattaini K, Sullivan M, Lau A, Fiske B, Bronson R, Vander Heiden M
BMC Cancer 2019;19(1)

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Hotspot SF3B1 mutations induce metabolic reprogramming and vulnerability to serine deprivation

Dalton W, Helmenstine E, Walsh N, Gondek L, Kelkar D, Read A, Natrajan R, Christenson E, Roman B, Das S, Zhao L, Leone R, Shinn D, Groginski T, Madugundu A, Patil A, Zabransky D, Medford A, Lee J, Cole A, Rosen M, Thakar M, Ambinder A, Donaldson J, DeZern A, Cravero K, Chu D, Madero-Marroquin R, Pandey A, Hurley P, Lauring J, Park B
Journal of Clinical Investigation 2019;129(11):4708-4723

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Genome-wide CRISPR/Cas9 library screening identified PHGDH as a critical driver for Sorafenib resistance in HCC

Wei L, Lee D, Law C, Zhang M, Shen J, Chin D, Zhang A, Tsang F, Wong C, Ng I, Wong C, Wong C
Nature Communications 2019;10(1)

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Translational and HIF-1α-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides

Hulea L, Gravel S, Morita M, Cargnello M, Uchenunu O, Im Y, Lehuédé C, Ma E, Leibovitch M, McLaughlan S, Blouin M, Parisotto M, Papavasiliou V, Lavoie C, Larsson O, Ohh M, Ferreira T, Greenwood C, Bridon G, Avizonis D, Ferbeyre G, Siegel P, Jones R, Muller W, Ursini-Siegel J, St-Pierre J, Pollak M, Topisirovic I
Cell Metabolism 2018;28(6):817-832.e8

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PHGDH as a Key Enzyme for Serine Biosynthesis in HIF2α-Targeting Therapy for Renal Cell Carcinoma

Yoshino H, Nohata N, Miyamoto K, Yonemori M, Sakaguchi T, Sugita S, Itesako T, Kofuji S, Nakagawa M, Dahiya R, Enokida H
Cancer Research 2017;77(22):6321-6329

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Modulating the therapeutic response of tumours to dietary serine and glycine starvation

Maddocks O, Athineos D, Cheung E, Lee P, Zhang T, van den Broek N, Mackay G, Labuschagne C, Gay D, Kruiswijk F, Blagih J, Vincent D, Campbell K, Ceteci F, Sansom O, Blyth K, Vousden K
Nature 2017;544(7650):372-376

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Hexokinases link DJ-1 to the PINK1/parkin pathway.

Hauser DN, Mamais A, Conti MM, Primiani CT, Kumaran R, Dillman AA, Langston RG, Beilina A, Garcia JH, Diaz-Ruiz A, Bernier M, Fiesel FC, Hou X, Springer W, Li Y, de Cabo R, Cookson MR
Molecular neurodegeneration 2017 Sep 29;12(1):70

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Validating and enabling phosphoglycerate dehydrogenase (PHGDH) as a target for fragment-based drug discovery in PHGDH-amplified breast cancer

Unterlass J, Baslé A, Blackburn T, Tucker J, Cano C, Noble M, Curtin N
Oncotarget 2016;9(17):13139-13153

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Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) and serine biosynthetic pathway genes are co-ordinately increased during anabolic agent-induced skeletal muscle growth

Brown D, Williams H, Ryan K, Wilson T, Daniel Z, Mareko M, Emes R, Harris D, Jones S, Wattis J, Dryden I, Hodgman T, Brameld J, Parr T
Scientific Reports 2016;6(1)

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A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate

Pacold M, Brimacombe K, Chan S, Rohde J, Lewis C, Swier L, Possemato R, Chen W, Sullivan L, Fiske B, Cho S, Freinkman E, Birsoy K, Abu-Remaileh M, Shaul Y, Liu C, Zhou M, Koh M, Chung H, Davidson S, Luengo A, Wang A, Xu X, Yasgar A, Liu L, Rai G, Westover K, Vander Heiden M, Shen M, Gray N, Boxer M, Sabatini D
Nature Chemical Biology 2016;12(6):452-458

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Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers

Mullarky E, Lucki N, Beheshti Zavareh R, Anglin J, Gomes A, Nicolay B, Wong J, Christen S, Takahashi H, Singh P, Blenis J, Warren J, Fendt S, Asara J, DeNicola G, Lyssiotis C, Lairson L, Cantley L
Proceedings of the National Academy of Sciences 2016;113(7):1778-1783

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High level PHGDH expression in breast is predominantly associated with keratin 5‐positive cell lineage independently of malignancy

Gromova I, Gromov P, Honma N, Kumar S, Rimm D, Talman M, Wielenga V, Moreira J
Molecular Oncology 2015;9(8):1636-1654

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Reducing the serine availability complements the inhibition of the glutamine metabolism to block leukemia cell growth

Polet F, Corbet C, Pinto A, Rubio L, Martherus R, Bol V, Drozak X, Grégoire V, Riant O, Feron O
Oncotarget 2015;7(2):1765-1776

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NRF2 regulates serine biosynthesis in non–small cell lung cancer

DeNicola G, Chen P, Mullarky E, Sudderth J, Hu Z, Wu D, Tang H, Xie Y, Asara J, Huffman K, Wistuba I, Minna J, DeBerardinis R, Cantley L
Nature Genetics 2015;47(12):1475-1481

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Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability

Markkanen E, Fischer R, Ledentcova M, Kessler B, Dianov G
Nucleic Acids Research 2015;43(7):3667-3679

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An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation

Mattaini K, Brignole E, Kini M, Davidson S, Fiske B, Drennan C, Vander Heiden M
Cancer & Metabolism 2015;3(1)

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p53 Protein-mediated Regulation of Phosphoglycerate Dehydrogenase (PHGDH) Is Crucial for the Apoptotic Response upon Serine Starvation

Ou Y, Wang S, Jiang L, Zheng B, Gu W
Journal of Biological Chemistry 2015;290(1):457-466

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Mouse Genetics Suggests Cell-Context Dependency for Myc-Regulated Metabolic Enzymes during Tumorigenesis

Clurman B, Nilsson L, Plym Forshell T, Rimpi S, Kreutzer C, Pretsch W, Bornkamm G, Nilsson J
PLoS Genetics 2012;8(3):e1002573

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Serine starvation induces stress and p53-dependent metabolic remodelling in cancer cells

Maddocks O, Berkers C, Mason S, Zheng L, Blyth K, Gottlieb E, Vousden K
Nature 2012;493(7433):542-546

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Functional genomics reveal that the serine synthesis pathway is essential in breast cancer

Possemato R, Marks K, Shaul Y, Pacold M, Kim D, Birsoy K, Sethumadhavan S, Woo H, Jang H, Jha A, Chen W, Barrett F, Stransky N, Tsun Z, Cowley G, Barretina J, Kalaany N, Hsu P, Ottina K, Chan A, Yuan B, Garraway L, Root D, Mino-Kenudson M, Brachtel E, Driggers E, Sabatini D
Nature 2011;476(7360):346-350