F48338

antibody from NSJ Bioreagents

Targeting: PHGDH PDG, PGDH, SERA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F48338

Provider

NSJ Bioreagents

Product name

PHGDH Antibody

Antibody type

Polyclonal

Description

This highly specific PHGDH antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the PHGDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of PHGDH antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (2). Predicted molecular weight ~56 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of PHGDH antibody and mouse brain tissue lysate. Predicted molecular weight ~56 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human brain tissue stained with PHGDH antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using PHGDH antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.