Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-87311 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87311, RRID:AB_11010147
- Product name
- Rabbit Polyclonal PHGDH Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PHGDH antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Metabolic Reprogramming by Dual AKT/ERK Inhibition through Imipridones Elicits Unique Vulnerabilities in Glioblastoma.
An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation.
NRF2 regulates serine biosynthesis in non-small cell lung cancer.
Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability.
Serine starvation induces stress and p53-dependent metabolic remodelling in cancer cells.
Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.
Ishida CT, Zhang Y, Bianchetti E, Shu C, Nguyen TTT, Kleiner G, Sanchez-Quintero MJ, Quinzii CM, Westhoff MA, Karpel-Massler G, Prabhu VV, Allen JE, Siegelin MD
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Nov 1;24(21):5392-5406
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Nov 1;24(21):5392-5406
An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation.
Mattaini KR, Brignole EJ, Kini M, Davidson SM, Fiske BP, Drennan CL, Vander Heiden MG
Cancer & metabolism 2015;3:5
Cancer & metabolism 2015;3:5
NRF2 regulates serine biosynthesis in non-small cell lung cancer.
DeNicola GM, Chen PH, Mullarky E, Sudderth JA, Hu Z, Wu D, Tang H, Xie Y, Asara JM, Huffman KE, Wistuba II, Minna JD, DeBerardinis RJ, Cantley LC
Nature genetics 2015 Dec;47(12):1475-81
Nature genetics 2015 Dec;47(12):1475-81
Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability.
Markkanen E, Fischer R, Ledentcova M, Kessler BM, Dianov GL
Nucleic acids research 2015 Apr 20;43(7):3667-79
Nucleic acids research 2015 Apr 20;43(7):3667-79
Serine starvation induces stress and p53-dependent metabolic remodelling in cancer cells.
Maddocks OD, Berkers CR, Mason SM, Zheng L, Blyth K, Gottlieb E, Vousden KH
Nature 2013 Jan 24;493(7433):542-6
Nature 2013 Jan 24;493(7433):542-6
Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis.
Nilsson LM, Forshell TZ, Rimpi S, Kreutzer C, Pretsch W, Bornkamm GW, Nilsson JA
PLoS genetics 2012;8(3):e1002573
PLoS genetics 2012;8(3):e1002573
Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.
Possemato R, Marks KM, Shaul YD, Pacold ME, Kim D, Birsoy K, Sethumadhavan S, Woo HK, Jang HG, Jha AK, Chen WW, Barrett FG, Stransky N, Tsun ZY, Cowley GS, Barretina J, Kalaany NY, Hsu PP, Ottina K, Chan AM, Yuan B, Garraway LA, Root DE, Mino-Kenudson M, Brachtel EF, Driggers EM, Sabatini DM
Nature 2011 Aug 18;476(7360):346-50
Nature 2011 Aug 18;476(7360):346-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PHGDH Antibody [NBP1-87311] - Simple Western lane view shows a specific band for PHGDH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PHGDH Antibody [NBP1-87311] - Electropherogram image(s) of corresponding Simple Western lane view. PHGDH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody [NBP1-87311] - Analysis in human cell lines SK-MEL-30 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody [NBP1-87311] - Analysis using Anti-PHGDH antibody NBP1-87311 (A) shows similar pattern to independent antibody NBP1-87310 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHGDH Antibody [NBP1-87311] - Analysis: NBP1-87311 (A) shows similar pattern to independent antibody NBP1-87310 (B)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal and glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human breast cancer shows moderate to strong cytoplasmic positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHGDH Antibody [NBP1-87311] - Immunohistochemical staining of human skeletal muscle shows no positivity as expected.